Re: [ccp4bb] problem with RELION particle picking

2025-03-11 Thread Takanori Nakane
Hi, Your file name contains space: "E1 11-2-25 URAC.tif". This is not allowed. Please remove space from file names. Also please don't use screenshots to show textual error messages. Copy and paste them as texts next time. Best regards, Takanori Nakane On 2025/03/11 22:18, Pree

Re: [ccp4bb] problem with RELION particle picking

2025-03-11 Thread Takanori Nakane
Hi, I cannot reproduce your problem. I did (with RELION 5.0): 1. Import: make sure to set "Are these multi-frame movies:" No. 2. Manual picking: select "Import/jobXXX/micrographs.star". Best regards, Takanori Nakane On 3/11/25 20:29, Preeti wrote: Hello every

Re: [ccp4bb] how to speed up polishing

2024-11-15 Thread Takanori Nakane
particles, not frames. Or could the problem be related to --only_do_unfinished issue discussed in: https://github.com/3dem/relion/issues/985    ? I believe Polish does not have this problem. Best regards, Takanori Nakane On 14.11.24 13:58, Takanori Nakane wrote: [EXTERNAL EMAIL - USE CAUTION

Re: [ccp4bb] how to speed up polishing

2024-11-14 Thread Takanori Nakane
ion of a job. In case of doubts, you can kill and continue the job. Best regards, Takanori Nakane On 11/14/24 21:49, Leonid Sazanov wrote: Hi, we are having some trouble finishing the polishing step. There are about 11k movies from K3 super-resolution pixel 0.53, motion-corrected in reli

Re: [ccp4bb] Relion issue with MPI

2023-12-22 Thread Takanori Nakane
particular dataset? A very similar issue was reported in https://github.com/3dem/relion/issues/1056 but I do not know what is the cause at the moment. Best regards, Takanori Nakane On 12/23/23 03:31, Srivastava, Dhiraj wrote: Hi I am trying to use relion and I am getting error when trying to use mpi

Re: [ccp4bb] Relion MotionCorr error

2019-06-17 Thread Takanori Nakane
e. > TIFFFetchDirectory: Can not read TIFF directory count. > TIFFReadDirectory: Failed to read directory at offset 152370858. These are real errors. Some of your TIFF files are corrupted (typically truncated). Look at the file sizes and remove those significantly smaller than the others.

Re: [ccp4bb] merging an hkl file

2018-04-18 Thread Takanori Nakane
hkl of CrystFEL. Don't use it. The documentations warn it. http://www.desy.de/~twhite/crystfel/manual-process_hkl.html http://www.desy.de/~twhite/crystfel/bestpractice.html Best regards, Takanori Nakane > What does snr mean? > Eleanor > On 18 April 2018 at 00:27, Gihan Ketawala wrote

Re: [ccp4bb] protein quasicrystals?

2018-02-13 Thread Takanori Nakane
Hi, "dials.reciprocal_space_viewer" is very useful to identify multiple lattices. For quasicrystal and modulated crystals, "dials.rs_mapper" is also very useful. Best regards, Takanori Nakane > Have you tried microseeding of these sphere crystals? It may help t

Re: [ccp4bb] proxy settings for ccp4 package manager

2018-01-10 Thread Takanori Nakane
Hi Johannes, On RHEL 6.7, setting "http_proxy" was sufficient. Best regards, Takanori Nakane On 2018/01/09 13:18, Johannes Cramer wrote: Dear CCP4bb, does anyone know how I can make the ccp4 package manager use a proxy for downloading in Linux 64bit versions? This would make inst

Re: [ccp4bb] paired refinement

2015-07-02 Thread Takanori Nakane
should be more accurate and "reliable" than that calculated from a dataset with multiplicity 2, but this difference is ignored in t-test.) Did anyone try this? Best regards, Takanori Nakane On 2015年07月03日 04:41, Tim Gruene wrote: Hi Robbie, I have been wondering how much information would

Re: [ccp4bb] Coot and Pymol through SSH by Xming/PUTTY on a windows client?

2015-07-01 Thread Takanori Nakane
Hi, I have successfully forwarded OpenGL applications like PyMOL using VirtualGL http://www.virtualgl.org/. Takanori Nakane On 2015年07月02日 14:09, Dale Tronrud wrote: Then I would worry that your local X11 server doesn't support glut. 3d graphics is an additional layer to the pro

Re: [ccp4bb] Cryo-EM

2015-05-19 Thread Takanori Nakane
Hi Faisal, Recordings of MRC-LMB EM-course last year are available at http://www2.mrc-lmb.cam.ac.uk/groups/scheres/impact.html Best regards, Takanori Nakane On 2015/05/20 2:36, Yi-Wei Chang wrote: Hi Faisal, Here is a new series of lecture video called "Getting Started in Cryo-EM&quo

Re: [ccp4bb] Reading coords and maps in COOT for PDB entries in mmcif format

2015-04-28 Thread Takanori Nakane
Dear Andrew, This is not very CCP4-way, but you can use: phenix.fetch_pdb --maps 4wz7 It took about 10 minutes on my PC. Best regards, Takanori Nakane On 2015年04月28日 18:35, Andrew Leslie wrote: I am trying to get coordinates and maps into COOT for a PDB entry that is only in mmcif format

Re: [ccp4bb] Absence of contact between layers in a crystal

2015-02-06 Thread Takanori Nakane
Hi, Another example is 2ZW3, again a membrane protein. Best regards, Takanori Nakane On 2015/02/06 21:11, Adrian Goldman wrote: Our recent membrane protein structure (4av3) doesn’t have much in the way of contacts either, as is characteristic of type 1 membrane protein crystals

Re: [ccp4bb] CCP4 Study Weekend 2015 "Advances in Experimental Phasing" - Live Web Streaming

2015-01-08 Thread Takanori Nakane
settings from 2014. Best regards, Takanori Nakane On 2015/01/08 19:34, Harry Powell wrote: I'm not sure the "what's new" sessions are webcast - try again when the main sessions are due to start at 11 am local time (in about 25 minutes time). Harry -- Dr Harry Powell, MRC L

Re: [ccp4bb] XDS orientation matrix inconsistent with Mosflm, DENZO

2014-12-28 Thread Takanori Nakane
Hi, xdsme has xds2mos.py script. https://code.google.com/p/xdsme/ https://code.google.com/p/xdsme/source/browse/XOconv/xds2mos.py Best regards, Takanori Nakane On 2014/12/29 6:37, Igor Petrik wrote: I am working on a small project which requires me to obtain the proper orientation of a

Re: [ccp4bb] Rfree set for twinned data

2014-07-07 Thread Takanori Nakane
e twin lattice (assuming the > data is twinned), obtain the same flag. This includes both the > possibility of merohedral and pseudomerohedral twinning. Best regards, Takanori Nakane (2014/07/07 7:31), Yamei Yu wrote: Hi all, Is there anything that needed to pay attention to while choosing R

Re: [ccp4bb] Off-topic PyMOL Issue

2013-03-02 Thread Takanori Nakane
Dear Greg, The following might work: set cylinder_shader_ff_workaround, on Best regards, Takanori Nakane

Re: [ccp4bb] Drawing reaction mechanism

2012-05-31 Thread Takanori Nakane
Hi, > Anyone please tell me > the name of programme which can be used to draw enzyme reaction mechanism ( > like Sn1 and Sn2). Your kind help will be much appreciated BKChem is also free software. http://bkchem.zirael.org/ Takanori Nakane

Re: [ccp4bb] Who is using 64-bit Linux?

2012-04-04 Thread Takanori Nakane
on registers, the faster it runs. Best regards, Takanori Nakane

Re: [ccp4bb] Iphone app to display protein models

2012-01-27 Thread Takanori Nakane
view is a nice viewer, too. http://www.molsoft.com/iMolview.html Best regards, Takanori Nakane P.S. For Android users, I am developing NDKmol. https://market.android.com/details?id=jp.sfjp.webglmol.NDKmol

Re: [ccp4bb] Software for showing crystal packing

2011-12-03 Thread Takanori Nakane
f you want to put it on Web pages, you can use Jmol. GLmol, a WebGL/Javascript based viewer I am developing, can also show packing and unit cell if symmetry operations are defined in REMARK record. http://webglmol.sourceforge.jp/index-en.html Takanori Nakane