Re: [ccp4bb] Removing a tight binding ligand

2010-10-08 Thread SIPPEL,KATHERINE H
n't see any mention on the subject in the archives. I >>> had hoped you might be able to point me in the right direction. >>> >>> Thanks for your time, >>> >>> Katherine >>> >>> Ph. D. candidate >>> Department of Biochemistry and Molecular Biology >>> College of Medicine >>> University of Florida >>> > > -- SIPPEL,KATHERINE H Ph. D. candidate Department of Biochemistry and Molecular Biology College of Medicine University of Florida

[ccp4bb] Removing a tight binding ligand

2010-10-07 Thread SIPPEL,KATHERINE H
Hi all, I am working with a substrate binding protein. The protein scavenges its endogenous ligand out of the E. coli used for expression. I need to get this ligand out for both crystallographic and kinetic studies. I have tried denaturing in urea and refolding the protein with limited succes

[ccp4bb] Two cells, one crystal

2010-08-03 Thread SIPPEL,KATHERINE H
Hi all, I work on a protein that has two know space groups at the same condition. One is P21 with 1 molecule in the ASU (small cell) and the other is P21 with 2 molecules in the ASU (large cell with pseudo-translational symmetry and is a giant pain in the butt to deal with). I was shooting a

Re: [ccp4bb] Modified Arginine Coot and SHELX

2010-06-23 Thread SIPPEL,KATHERINE H
s case). Coot runs libcheck for you. Paul. -- SIPPEL,KATHERINE H Ph. D. candidate McKenna Lab Department of Biochemistry and Molecular Biology College of Medicine University of Florida

Re: [ccp4bb] Cut ligand or keep in whole piece when only part of it can be resolved

2010-05-18 Thread SIPPEL,KATHERINE H
can be identified. Is it ok for me to cut ligand 1 so only the identifiable part is left or should I keep the whole piece? Thanks in advance. Jay -- SIPPEL,KATHERINE H Ph. D. candidate McKenna Lab Department of Biochemistry and Molecular Biology College of Medicine University of Florida

Re: [ccp4bb] Micro-g Crystal Growth and the literature

2010-05-10 Thread SIPPEL,KATHERINE H
ld be a small corner of a niche market. HTH Henry Bellamy Associate Professor - Research Center for Advanced Microstructures and Devices Louisiana State University 6980 Jefferson Hwy. Baton Rouge LA 70806 225-578-9342 (voice) 225-578-6954 (fax) hbell...@lsu.edu -- SIPPEL,KATHERINE H Ph. D. c

[ccp4bb] To model or not to model...

2010-04-13 Thread SIPPEL,KATHERINE H
ut I would genuinely like to know what people think. Thanks, Katherine Sippel SIPPEL,KATHERINE H Ph. D. candidate Department of Biochemistry and Molecular Biology College of Medicine University of Florida

Re: [ccp4bb] SAD phasing at home source

2009-10-26 Thread SIPPEL,KATHERINE H
gen Interfakult??res Institut f??r Biochemie Dr. Georg Zocher Hoppe-Seyler-Str. 4 72076 Tuebingen Germany Fon: +49(0)-7071-2973374 Mail: georg.zoc...@uni-tuebingen.de http://www.ifib.uni-tuebingen.de -- SIPPEL,KATHERINE H Ph. D. candidate Department of Biochemistry and Molecular Biology