Hi Jay,
Are you sure the missing part of the ligand is disordered and not
cleaved? Depending on how precious the enzyme and the ligand are,
it might be worth denaturing the crystals, centrifugally filtering
the unfolded protein and running mass spec on the flow-through.
My vote is build what you see for what it is worth.
Cheers,
Katherine
On Tue May 18 09:44:59 EDT 2010, Jay Pan <ccp4p...@gmail.com>
wrote:
Hi everyone,
I have two types of ligands cocrystallized with my protein,
ligand 1 and ligand 2. I am only interested in resolving ligand
2. In my electron density map, only part of ligand 1 can be
identified. Is it ok for me to cut ligand 1 so only the
identifiable part is left or should I keep the whole piece?
Thanks in advance.
Jay
--
SIPPEL,KATHERINE H
Ph. D. candidate
McKenna Lab
Department of Biochemistry and Molecular Biology
College of Medicine
University of Florida
