tRNA should be very easy to see. I suggest to buy cheap yeast tRNA as
control and test different concentrations and different dyes until you can
see it on the gel. I am not sure about SybrGold. It is a tricky dye and may
take a long time to get in. Filter and excitation both need optimization.
If
han
24 hours are needed.
I’d be happy to discuss further.
Best of luck,
Gabe Salzman
On Sat, Jan 31, 2015 at 11:46 AM, Nick Huang wrote:
> Hi all,
> I started a crank 2 process to run a SAD model building with a partial
> model input yesterday. From the log file, crank 2 was in the s
tput. Does anybody know if
it is normal or there is an error?
Thanks,
Nick Huang
Hauptman-Woodward Institute
density modification is better, but to run the final
refinement with full dataset instead because refinement also treats
unisotropicity?
Best,
Nick Huang
Yes, it is very common. I solved two of them at 2-3 A resolution and
saw my colleague had one with 1.4 A resolution (can't remember the
name of the protein).
Please see references
J Med Chem. 2010 Jul 22;53(14):5229-39. doi: 10.1021/jm100377f.
Complexes of bacterial nicotinate mononucleotide aden
