Dear all, I am trying to figure out what exactly Autoscale does in HKL2000 and decide what is the best practice. Autoscale definitely does more than adjusting error models for me. It seems that using the .sca file it generated dramatically improved my density modification map. However, Xtriage from phenix suggested that the data was truncated or scaled for its unisotropicity and data completeness suffered. As a result it suggested me to use a full dataset instead and let the refinement take care of the unisotropicity. Maybe I should use the autoscaled dataset to build the model because the density modification is better, but to run the final refinement with full dataset instead because refinement also treats unisotropicity?
Best, Nick Huang
