Hi Gabriel, My datasize is very small with a very low resolution. Was there an error output when your refmac process didn't converge? I started a SAD process along as you suggested and will see what is going to happen. Thanks
NIck >>>>>> Hi Nick, In my experience, this is too long and there is probably an error. That said, the SAD dataset I used for crank 2 with a partial model was small (~18,000 reflections and no NCS at 3Å). Interestingly, though I had a partial model (built with phenix.mr_rosetta), crank 2 failed to converge when I gave it this model (took ~3-4 hours to finish on a dual core 2.5Ghz iMac). When I ran crank 2 with my SAD dataset alone (no partial model input), I got a much better solution in ~2 hours and was able to solve the structure, so I suggest you try this as well. Depending on your computer and dataset size, it is possible that more than 24 hours are needed. I’d be happy to discuss further. Best of luck, Gabe Salzman On Sat, Jan 31, 2015 at 11:46 AM, Nick Huang <huangn...@gmail.com> wrote: > Hi all, > I started a crank 2 process to run a SAD model building with a partial > model input yesterday. From the log file, crank 2 was in the step of > "Starting > process of iterative refinement and (substructure) atom picking" and had > been running a whole night without any further output. Does anybody know if > it is normal or there is an error? > Thanks, > Nick Huang > > Hauptman-Woodward Institute > >
