[ccp4bb] Glutathione sepharose

Hello, For various reasons we are frequently expressing proteins with a GST tag. The glutathione sepharose beads that we are using for affinity purification seem to be difficult to regenerate and we see much lower capacity when used the second time. We are following the manufacturer's instr

[ccp4bb] mtzdump missing?

I just downloaded the CCP4 6.1.13 version for Windows and attempted to run MolRep. After pointing to the mtz file I am getting the message that mtzdump is missing. Have to kill to GUI to get out of it. Where is the code for mtzdump? Best,     Mirek

[ccp4bb] Idealizing helix

I am looking at a protein model (no electron density) that has a highly distorted helix. I would like to idealize this helix in the context of the protein, e.g. do least squares idealization with restrains on the backbone torsion angles, with the smallest possible divergence from the initial struct

[ccp4bb] Post-doctoral position in Montreal

Several post-doctoral positions in the area of structural biology are available in the laboratory of Dr. Mirek Cygler and funded by CIHR. The research activities of the group focus on structural and functional characterization of proteins to gain detailed understanding of their mode of

[ccp4bb] question

Hi, Here is a question from a Windows illiterate. I would like to run pdbcur with parameters that are not accessible through the CCP4i interface. Need to create symmetry-related molecule. I can modify the script file but I do not know how to execute the program. I guess that I only know how