Re: [ccp4bb] Multiple binding model in BLI

2024-08-28 Thread Mintu Chandra
do anything you > ever want to do with transit and equilibrium kinetics analysis. > > A. > > > > > On 28 Aug 2024, at 17:16, Mintu Chandra wrote: > > *LET OP:* Deze e-mail is afkomstig van buiten de organisatie. Open alleen > links of bijlagen als je de afzender ken

[ccp4bb] Multiple binding model in BLI

2024-08-28 Thread Mintu Chandra
Hello everyone, I am trying to fit a BLI sensogram curve into a 1:3 binding model but there is limited scope in the software package (limited to 1:2 binding model) and therefore not able to fit the curve to get the accurate KD value. I am trying graphpad prism but not getting enough information on

[ccp4bb] AutoSol invalid MTZ column_types error

2017-06-07 Thread Mintu Chandra
Dear all, I am getting an error message "Invalid MTZ column_types for the given miller_array." while I try to run AutoSol. The last line in the log is "Adding array ['N(+)', 'N(-)'] to output file with type II". Do you have any suggestion how to fix this error? Thanks a lot in advance. Best, Mi

[ccp4bb] relative domain motion calculation...

2015-04-21 Thread Mintu Chandra
Dear All, I am working with a protein containing tandem domains, connected with 3-4 residue linker. I want to calculate the relative motion/orientation of one domain with respect to the hinge region as well as with respect to the other domain. Both the domains have very less seq. identity (<20%). P

[ccp4bb] secondary structure problem...

2015-01-14 Thread Mintu Chandra
Dear All, I am refining one of the structure at 3.1 A resolution with R factor and R free of 30 and 34 with setmet peak data. Some of the region in the model look like loops without any secondary structure (helix and strands). Can anybody help me out ??? Mintu.

[ccp4bb] protocol for seleno-methionine incorporation...

2014-06-23 Thread Mintu Chandra
should use the purified protein or digested protein for mass spectrometry. Thanks, Mintu Chandra. -- Mintu Chandra Senior Research Fellow IISER BHOPAL mi...@iiserb.ac.in +918085288853

[ccp4bb] Align linux version

2013-11-10 Thread Mintu Chandra
Dear CCP4 users, We are trying to align two different structures. It would be helpful for us if anybody has linux compiled align version? Regards, Mintu Chandra