[ccp4bb] Off-topic looking for unusual expression promoter

NOT require cAMP/CRP/CAP binding in the bug for expression? I can't find anything yet by lit. searches. Thanks Laurie Betts

Re: [ccp4bb] Unexplained negative fo-fc

My experience is also that the bulk solvent model sometimes makes the mask exclude internal large cavities. Laurie Betts On Fri, Apr 12, 2013 at 12:03 PM, Pavel Afonine wrote: > P.S.: you are not alone having such R-factors > > On Fri, Apr 12, 2013 at 9:50 AM, Jan van Agtho

[ccp4bb] another mystery density (link to picture no attachment)

sausage density? Laurie Betts https://docs.google.com/file/d/0Bx8-qQA_HKegekZKSng1ZEV2MHM/edit

Re: [ccp4bb] Today ...

OMG LOL Geeks! Dog bless you all!!! On Thu, Dec 20, 2012 at 8:52 PM, Bosch, Juergen wrote: > May I introduce you to another fool proof way: > 12d12 > year-month-day > This is in particular useful in a mixed lab with american and europeans > labeling Eppendorf tubes differently. The month has a

Re: [ccp4bb] vitrification vs freezing

the duration of data collection." Laurie Betts On Thu, Nov 15, 2012 at 1:58 PM, Nukri Sanishvili wrote: > Hi Ethan, > > I am not a linguist of Greek or even of English but I would assume that > the term "cryo-cooling" is advocated not by DRD but by the people who wan

Re: [ccp4bb] Professor Dame Louise Johnson

What a great lady to have inspired so many, and to remind us how welcoming the field of X-ray crystallography has been in general for women because of people like Dr. Johnson, Dorothy Hodgkin, and Rosalind Franklin, and many others. On Tue, Oct 2, 2012 at 12:03 PM, Gloria Borgstahl wrote: > This

[ccp4bb] Areaimol under CCP46.3.0 under Windows 7 crashes

Dear ccp4, WE installed ccp4 6.3.0 GUI under Windows 7, and tried to run AREAIMOL on a single coordinate file with protein and some nucleic acid. It crashes with a "child killed segmentation violation" error, however the exact same input coordinate file runs fine on ccp4 6.3.0 under MacOSX. So i

Re: [ccp4bb] co-express two proteins in E.coli

Nickel affinity column to capture the complex (unpublished). Laurie Betts UNC Chapel Hill On Mon, Jul 16, 2012 at 11:55 PM, Biswajit Pal wrote: > I fully agree with Dima. We are able to co-express and purify two > interacting partners using pET28 and pET21 in E. coli. Some related > refer

[ccp4bb] npr videos of crystals growing

to make something like that of a protein crystal growing. Wouldn't that be fun to see/hear about on NPR!! http://www.sciencefriday.com/feeds/video/ Cheers Laurie Betts

[ccp4bb] help offline for cns 1.3?

Sorry - I already belong to 2 bbs and to add another would make me really a mess. Can someone out there who is a CNS developer please send me an email off this bb I need to ask a glibc question. Thanks, Laurie Betts laurie.betts0...@gmail.com

Re: [ccp4bb] Protein preps become a jelly

I have had protein crystals (so very high protein concentration) that turn into gummy bear-like objects, where instead of crumbling they are like, well, a gummy bear or a piece of rubber. I attributed it to oxidation or other chemical ageing processes. I am sure others will have suggestions for p

Re: [ccp4bb] Question about TEV cleavage

We have the same experience with the DO NOT AGITATE. We purify our own His-tagged TEV protease, and flash-freeze it IMMEDIATELY after IMAC prep at about 1 mg/mL. Laurie Betts UNC On Thu, Mar 31, 2011 at 10:37 AM, Charles Allerston < charles.allers...@sgc.ox.ac.uk> wrote: > I

[ccp4bb] expression of Cys-rich small protein

mM DTT or TCEP).Thinking of trying chaperones and insect cells next. Any experience out there that might help? Mostly I wonder about all the cysteines. Don't really know if that is the problem. Laurie Betts

Re: [ccp4bb] Too many microcrystals.......

Hi Dilip- Try using your microcrystals as seeds and try the matrix microseeding method: Acta Crystallogr D Biol Crystallogr.2007 Apr;63(Pt 4):550-4. Epub 2007 Mar 16. An automated microseed matrix-screening method for protein crystallization. D'Arcy A

[ccp4bb] COOT install issue

The coot we installed on CentOS Linux having a problem opening .mtz files. The program starts and opens coordinate files. THis is the error message "ERROR: using display list 0 when main_or_secondary is 0" I tried both Auto open and manually open .mtz THanks

[ccp4bb] Schroder et al. DEN potential

e but I'd like to try! I should probably have posted this to CNSbb but I don't currently belong- sorry. Thanks Laurie Betts UNC

Re: [ccp4bb] Re： [ccp4bb] Mysterious Crystals?

Dear Cheng, You could take your non-diffracting needle urchins and crush them in their growth solution (vortex several minutes and/or try Hampton seed bead). Then make serial dilutions of the seed solution and add them to a whole new screen (the MMS method) or try the optimization method with new

[ccp4bb] cryoprotection for fragile crystals

I found within the last few years a nice flowchart that someone (I think it was Artem Evdokimov) had posted somewhere for a protocol for dealing with crystals that you couldn't just quick dip, and now I can't find it - maybe he can send it to me offbb??? Thanks Laurie Betts UNC CHapel Hill

[ccp4bb] X-ray Facility Manager Position: University of Pittsburgh

At the risk of being the first job posting after the recent discussion, please see the following link for job description: http://www.structbio.pitt.edu/webusers/xray/ -- Laurie Betts X-ray Crystallography Facility Manager Department of Structural Biology University of Pittsburgh 1050 BST3 3501

[ccp4bb] how do you specify Heme oxidation state?

n that was originally purged with CO but the ligand looks more like OH. But the amout of residual density around the Fe atom changes depending on what ligand we put into the refinement. even though we always use the HEM.cif. Yeesh... THanks Laurie Betts University of Pittsb

[ccp4bb] how to run detwin?

I have a P62 crystal that is probably p31 (or P32). When I run the detwin program to actually output detwinned data, do I input the mtz as processed (e.g. as output from dtrek or HKL2000) in the wrong (higher symmetry) or the correct space group - Thanks Laurie Betts University of

[ccp4bb] negative difference density in protein interiors

Specifically, hemoglobin, in original PHASER (unrefined) solution DELFWT an continuing to show up in refined DELFWT coefficients. But I have seen it in other cases too. What does it mean? Thanks Laurie Betts University of Pittsburgh X-ray Crystallography Facility Manager Department of

[ccp4bb] more on lattice translocation defects

can give a definition in terms that is simple (for my simple mind). Thanks -- Laurie Betts X-ray Crystallography Facility Manager Department of Structural Biology University of Pittsburgh 1050 BST3 3501 Fifth Ave Pittsburgh, PA 15260 412-383-5839

[ccp4bb] Postdoctoral opportunities at University of Pittsburgh

a plus. Please direct inquiries; or send your CV and the names and contact information for three references to Ms. Janet Zambotti (NMR) or Dr. Laurie Betts (X-ray crystallography) to the following e-mail address: Laurie Betts X-ray Crystallography Facility Manager Department of Structural