I would warn you that the K3 and K9 chillers have internal hoses which cannot
tollerate water pressure above 125 psi. We had a hose fail from the building
chilled loop pressure. So, be careful adding any pressure with a pump before
the chiller...and maybe consider one after the chiller to pull
Additionally, once the crystals are ready for the trip, remember to place a
heat sink in with the samples. Planes and tarmacs are seldom at room
temperature.
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Bernard
If Paratone oil is too viscous, I recommend perfluoropolyether (PFPE). A lot
of small molecule groups use this because it reduces damage to the crystals.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Kelly Daughtry
To
If either of the two protein structures has been determined/deposited, I would
check if your unit cell matched one of them.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: "Tanner, John J."
To: CCP4BB@JISCMAIL.AC.UK
Se
You may want to check that the distance, 2-theta and wavelength are correct.
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: "herman.schreu...@sanofi.com"
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tue, September 11, 2012 1:29:26 A
I would contact Cole-Parmer (http://www.coleparmer.com/) and see what they
recommend. They have been very helpful in the past.
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
From: Georg Zocher
To: CCP4BB@JISCMAIL.AC.
When using oil on protein crystal mounts I suggest:
1. once the crystal is under oil, remove as much adhered solution from the
crystal surface.
2. if there are any volatile components in your drop condition, presaturate
the
oil with that solvent.
3. consider using perfluoropolyether since it
Have you tried adding water to your reservoir and allowing it to vapor diffuse
into the drop?
Kris
Kris F. Tesh, Ph. D.
Department of Biology and Biochemistry
University of Houston
- Original Message
From: Tim Gruene
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, February 28, 2011 3:48:25 AM
When I researched this a number of years back, both Taylor-Wharton and
Cole-Parmer techs said nearly all dewar failures were in the epoxy in the neck
of the dewar. This could not be remedied through pumping down the vacuum
jacket as the seal was breached.
They did give some advice to elongate
I know of one bad experience with a common refrigerator as it pertains to the
freezer. The refrigerator was great for the 4 deg crystallization trials, but
the freezer had a "frost-free" cycle which was destroying protein and
chemicals. The user was keeping lots of their sensitive items in the
Here is a start. It can imply you:
1. did not rotate through the complete unique angle to obtain all reflection
intensities.
2. had the detector too close so did not record reflections which were outside
the detector area. (extending the data resolution beyond the
detector/experimental geomet
I have carried Greiner plates all over the world with no problems, even when
asked what they were. I usually place them in a padded envelope (for
insulation) inside my back pack.
On the other hand, Limbro plates have been a little suspect. I have carried
them in styrofoam boxes, metal lunch b
I must agree that the less stressed part of a needle may be the end not
contacting the loop or meniscus, but protrudes out the end/top. One can
carefully manipulate the crystal so that it is not parallel with the pin either
by having a bent loop or setting the crystal slightly diagonal to the
Hey there Chris,
Additionally, I have seen trays set up under Argon atmosphere in glove
bags. They are sealed and left in the bag until inspection where they are
temporarily brought out and returned.
Then, when harvesting, they would use a glass dish (taller than the tray
plus some working a
ost.
Kris
-
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX 77381 USA
001 281 362 2300 x 144
From:
Kris Tesh
To:
CCP4BB@JISCMAIL.AC.UK
Date:
09/01/2009 04:20 PM
Subject:
[ccp4bb] Fw: [ccp4bb
.
Enjoy,
Kris
-
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX 77381 USA
001 281 362 2300 x 144
- Forwarded by Kris Tesh/Rigaku on 09/01/2009 03:25 PM -
From:
Kris Tesh/Rigaku
To
...and I prefer to use PFPE (perfluoropolyether, trade name FOMBLIN) as an
oil since it is less viscous, has no reactivity and is a low temp
lubricant that freezes easily. Small molecule crystallographers have used
it for years.
Kris
-
Kris F. Tesh, Ph D
Directo
A trick that small molecule crystallographers use is to make a packing
diagram where the volume includes both molecules A and B, or maybe even
two of each. In this case, it would display all of C (or maybe even 2 of
C either twisted back on itself (symmetry element near molecule C) or
extendin
The obvious difference is that the ratio of drop volume to well volume can
be potentially greater in non-microbatch wells, and can accommodate higher
solvent volume transfers...which is generally in dehydration, but can be
for hydration too.
Other considerations are the slower rate of temperatu
The Free Mounting System (FMS) from Proteros Biostructures can also be a
tool for this. As long as it does not have highly volatile components in
the crystalization conditions, room temperature testing is not too
difficult. There are other suppliers of humidity control devices which
are avail
At the risk of sounding too commercial, here are some suggestions:
1. See if you can either place your crystallization tray in a cold room or
crystallize it there. Mount your crystal in a capillary here so that at
whatever lower temperature you use, the distillation of solvent will be
reduced.
Dear Junhua,
Try this simple experiment...put a MiTeGen loop up without crystal or
solution and take am image. (...and then tell us what it looks like)
Kris
Kris F. Tesh, Ph D
Director, Macromolecular Products
Rigaku Americas Corporation
9009 New Trails Drive
The Woodlands, TX 7738
A less viscous oil used in small molecule low-temp work is
PerFluoroPolyEther. Its general use is as an ultra-high vacuum pump oil, so
it has an extremely low volatility. Additionally, PFPE has a very low
fracture temperature and is non-reactive. I have used it in both small
molecule and macromo
Although many of you may already use this technique, a more obvious, cheaper
and easier method is to just leave the cryovial (or puck) in the liquid
nitrogen while mounting your crystal. Once mounted, raise the vial out of
the dewar (slightly above the mouth of the dewar where the cooled gas is)
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