Dear Junhua, Try this simple experiment...put a MiTeGen loop up without crystal or solution and take am image. (...and then tell us what it looks like) Kris
------------ Kris F. Tesh, Ph D Director, Macromolecular Products Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA 001 281 362 2300 x 144 -----Original Message----- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Mark Agacan Sent: Tuesday, May 29, 2007 3:49 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] crystal shipping at room temperature Dear Junhua, This does indeed sound like a description of a fiber diffraction pattern. For example, as you probably already know, A-form DNA fibres give large bands close to 3.4 Angstroms at the top and at the bottom of the images. The exact positions of the bands are specific to the type of fibre. I don't know what fibres diffract to 4.8 - 5.5 Angstroms. I have never used MiTeGen mounting tools, so I can't comment on that. M >Another question about the MiTeGen mounting tools is that I always observe a stong diffraction ring at about 5 A. Well, it is not exactly a ring; it's actually two thick arches (pretty thick, roughly from 5.5 A to 4.8 A), one at the top and the other at the bottom of the diffraction patterns (nothing on the left-hand or right-hand side). Does anybody have any idea what this might be (fiber?)? > >thanks a lot for your help! > >Junhua >--- >Junhua Pan >Department of Biochemistry & Cell Biology >327 Keck Hall, Rice University >6100 Main Street MS-140 >Houston, TX 77005 >Phone: (713)348-3346 >Email: [EMAIL PROTECTED] > > _____________________________________ Dr Mark Agacan Scientific Officer, Division of Biological Chemistry and Molecular Microbiology, Wellcome Trust Biocentre, College of Life Sciences, Dow St., University of Dundee, Dundee, DD1 5EH Tel: +44 1382 388751 Fax: +44 1382 345764 _____________________________________
