The obvious difference is that the ratio of drop volume to well volume can be potentially greater in non-microbatch wells, and can accommodate higher solvent volume transfers...which is generally in dehydration, but can be for hydration too.
Other considerations are the slower rate of temperature change with larger volumes, the distance between the drop and reservoir, rate of drop dehydration when opened, and (for practical use) the ease of withdrawing crystals. And, although many crystals show evidence of crystallization in both systems, some will only grow in one or the other. Kris --------------------------------- Kris F. Tesh, Ph D Director, Macromolecular Products Rigaku Americas Corporation 9009 New Trails Drive The Woodlands, TX 77381 USA 001 281 362 2300 x 144 From: rui <ruis...@gmail.com> To: CCP4BB@JISCMAIL.AC.UK Date: 04/22/2009 11:09 AM Subject: [ccp4bb] microbatch vs hanging drop Hi, I have a question about the method for crystallization. With traditional hanging drop(24 wells), one slide can also hold for multiple drops but it requires the buffer quite a lot, > 600uL? Microbatch can save buffers,only 100uL is required, and also can hold up to three samples in the sitting well. Other than saving the buffer, what's the advantage of microbatch? Which method will be easier to get crystals or no big difference? Thanks for sharing. R
