Re: [ccp4bb] Help crystalizing protein

Hi, three things come to mind besides what has been mentioned already - many proteins that have an affinity for nucleic acids actually love sulfates or phosphates… And yes, phosphates may lead to salt crystals at higher pH values but this should not stop you a priori from screening with the sam

Re: [ccp4bb] enzyme or other protein with multiple cofactors

Hi, cytochrome P450 (heme + nadp) nitric oxide synthase (FAD, FMN, heme) ferredoxin-NADP+ reductase succinate dehydrogenase complex II (FASD + iron-sulfur) ribonucleotide reductase (Fe + Mn + tyrosyl radical) lactate oxidase (FMN + Ca) glutamate synthase dihydrooroate DH Lipoamid DH Xanthine oxida

Re: [ccp4bb] 图片1

Hi, to me it looks like 2 things are happening in the drops, to many nuclei and on these initial nuclei new crystals appear. This may indicate that your protein is not that soluble to start with and that there may be some protein aggregation going on in your protein stock solution. Diluting the

Re: [ccp4bb] Apple M4 chip

Hi, compared to the M3, the upgrade is mainly about in the number of total cores, ratio between efficiency vs performance cores (6 + 3 or 4), 10 instead of 8 GPU cores, improved neural engine, increased maximum memory capacity (32GB) and 20% increased memory bandwidth (now 120 GB/s). Same is tr

Re: [ccp4bb] Co-Crystallization with drug molecule

Hi Amit, the questions and suggestions by Eta are important ones and I would like to extend them a little bit. For your apo crystals, instead of adding DMSO in one go, transfer the crystal to a drop with 5% DMSO and wait, if it survives, transfer to 10%, to 20% and finally to 30%. Then, do no

Re: [ccp4bb] Ligand identification in X-ray density

7229 Office: 706-583-0304 Lab:706-583-0303 FAX: 706-542-1738 *** From: Jeroen Mesters Date: Wednesday, June 5, 2024 at 1:17 PM To: Zachary A. Wood Cc: CCP4BB@jiscmail.ac.uk Subject: Re: [ccp4bb] Ligand identification in X-ray density You don

Re: [ccp4bb] Ligand identification in X-ray density

Original Message On 05/06/2024 17:54, Jeroen Mesters <cf8d8aa45b08-dmarc-requ...@jiscmail.ac.uk<mailto:cf8d8aa45b08-dmarc-requ...@jiscmail.ac.uk>> wrote: Thank you for this intriguing information! At the same time, this also implies that not all entities that have

Re: [ccp4bb] Ligand identification in X-ray density

logy University of Georgia Life Sciences Building, Rm A426B 120 Green Street Athens, GA 30602-7229 Office: 706-583-0304 Lab:706-583-0303 FAX: 706-542-1738 *** From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK>> on behalf of Jero

Re: [ccp4bb] Ligand identification in X-ray density

Hi, proteins my pick up ligands from the „source" from which they were isolated…. Looks to me like trimethylglycine, an amino-acid derivative found in plants… Regards, Jeroen __ https://orcid.org/-0001-8532-6699 Am 05.06.2024 um 17:44 schrieb Khadijah Ameen Khan : Dear All, I am a first

Re: [ccp4bb] E. coli strain M15 or SG13009

Hi Mark, Gentauer may still sell those… https://maxanim.com/strains/m15-prep4-escherichia-coli-strains/ Cheers, Jeroen __ Dr. math. et dis. nat. Jeroen R. Mesters Biological Safety Officer (BBS) Deputy, Lecturer, Program Coordinator Infection Biology Visiting Professorship in Biophysics South B

Re: [ccp4bb] Modeling Disulfide Bond Occupancies

Do not know what the resolution is here, try an anisotropic refinement of the Cys side chains first to see if this solves your problem. __ Dr. math. et dis. nat. Jeroen R. Mesters University of Lübeck https://orcid.org/-0001-8532-6699 Am 06.05.2024 um 21:48 schrieb Eleanor Dodson <176a9d

[ccp4bb] coot-0.5.2-osx-universal.dmg.gz

I am looking for coot-0.5.2-osx-universal.dmg.gz Anybody out there that can help? - J. -

Re: [ccp4bb] Summary of Thermofluor

mesters wrote: > Sorry for the off-topic but can somebody recommend highly a sensitive > RT-PCR machine for the thermofluor experiment (sypro orange). > That would imply excitation below 500 nm (ideally 470) and detection > at about 570 nm, right? I know several simple machines have a problem > wit

Re: [ccp4bb] WG: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

Hi Gregor, I think this LCD monitor is not useful since the refresh rate is only 75 Hz, thus in stereo 2 x 37.5 Hz and that is going to give a big headache. I know Samsung is working on a 100 Hz LCD-TV. Nevertheless, the goal is not for stereo but to suppress the afterglow effects again. They tri

Re: [ccp4bb] 3D Glasses - Vuzix HMD by eDimensional

Dear Pedro, there is no cheap solution for now, the 3D community has to wait for a few years more I think to be presented with a good stereo-capable LCD. The problem is not the refresh rate (as low as 5 ms nowadays) but it is the after glow effect... that is one of the reasons why LCDs provide a s

Re: [ccp4bb] protein degradation?

Hi, if I recall this correctly, it is the nickel that is in your sample after elution and boiling your protein in SDS sample buffer does the rest. So, could be the sample is fully okay!!! J. Tiago Botelho wrote: > Hi, > > I also had a similar problem with one of my proteins... I had it clon

Re: [ccp4bb] need help for viewing hardware stereo

Venky, have a look at your Xorg.config file in /etc/X11 directory. Make sure to set the options correct (Option "Stereo" "3")!: Section "Device" BoardName"Quadro FX 3450" BusID"1:0:0" Driver "nvidia" Identifier "Device[0]" Option "IgnoreEDID" Option "S

Re: [ccp4bb] stereo, dual head, CRT and TFT, Xorg.config, linux

For Stereophiles and Xineramaphiles, for those that asked me for our Xorg.config file (see below) for running a two screen setup (requires Nvidia Quadro card). This file is set up for a stereo beamer and a LCD. You have to do some editing of your xorg.conf file (do that by creating a copy and

[ccp4bb] Several positions available in Luebeck, Germany

Applicants interested in one of the positions listed below are invited to send their complete resumé (indicating the position of interest and including the names of three possible referees), before April 16, 2007, to: Universität zu Lübeck Dezernat Personal Ratzeburger Allee 160 23538 Lübeck Furt