mesters wrote: > Sorry for the off-topic but can somebody recommend highly a sensitive > RT-PCR machine for the thermofluor experiment (sypro orange). > That would imply excitation below 500 nm (ideally 470) and detection > at about 570 nm, right? I know several simple machines have a problem > with the 570 nm... > > Jeroen. > Many thanks to all that commented to my query!
A list of RT-PCR machines that match the specifications (ex. 470 and det. 570 nm (!), melting curve measurement possible, listed according to price) are: a) Biorad MiniOpticon b) Stratagene Mx3005p c) Biorad Biorad iQ5 and Aplied Biosystems StepOnePlus Other RT-PCR machines (not exactly the right detection filter but sufficient signal in the channel: listed according to price): a) Aplied Biosystems StepOne b) Biorad Biorad myiQ (rumors say it can be supplied with the correct filters. If so, it is slightly cheaper than the Mx3005p but clearly less flexible) The Stratagene Mx3005p (beware, the Mx3000p is not useful at all) is the most flexible machine for the money (5-channel detection among other things) and has been used successfully used with Sypro orange. Also the Biorad machines (iQ5 but also MiniOpticon) have a respectable reputation. Looks like the Biorad MiniOpticon (2-channel machine) will fit my budget but I must test the Applied Biosystems StepOne (3-channel machine, best offer) before making the final decision. This has to do with the fact that if the detection filters have a small band-path, a machine with an off-peak but broad band-path filter may detect more signal at the end of the day. - J. -
