I have had success with the standalone Coot, found here:
http://scottlab.ucsc.edu/~wgscott/xtal/wiki/index.php/Stand-Alone_Coot
It doesn't use the Fink package, which seems to be part of your problem. Try
completely uninstalling your version and reinstalling this one. I've had no
problems at
We recently had this problem in our lab.
No matter what we tried, we could not get convert2mtz (or any other program) to
work properly. We were probably doing something wrong with the fortran?
Depending on how far along you are, you can try using phenix.refine. Just
input your model and
I second autoSHARP/SHARP. It makes great initial maps, and once you get it
running, it is totally worth it.
Hello all,
Does anyone know of an example where SHARP was used to phase multiple
sulphur-SAD datasets and combine all of the data into one map?
I have multiple crystals of the same protein, but each crystal form has a
different LtoM mutation (the sulfur content of the native construct is too lo
Hi,
If I were you, I would collect a redundant dataset (~15-20 or even higher if
possible) at home and use the anomalous flag in Scalepack/Denzo. You should
be able to pick up the anomalous differences (especially with data to 2.0A)
for Se, even at CuKa wavelength at home!
Good luck!
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Hello all,
Does anyone know of some relevant literature (or a general idea really) that
discusses protein crystal solvent content vs. average resolution? I am
under the impression that the higher the solvent content, the lower the
average resolutionbut I'd like to see some sort of data to ba
Hello all,
I posted here a few months ago. My post dealt with a protein crystal that
was very sensitive to oxidation (with the SeMet derivative even more so).
The protein grows in 24% PEG 1500, no more than 1 mM DTT, 0.05 M Hepes, pH
7.5, 1% glycerol. TCEP will not work, even at very low con
Hello all,
Anyone have any tips for reducing agents for use with the following
crystallization conditions:
100 mM hepes, pH 7.5, 24% PEG 1500
or
100 mM Tris, pH 8.0, 24% PEG 1500
I've tried BME and DTT (1 mM). Currently trying out TCEP. The protein
seems to be sensitive to oxidation, with
This happened to me previously, and the only thing that worked for me was to
lower the protein concentration. I was setting my protein up at 10 mg/mL,
only to find skins on nearly all the drops. I would damage the crystals
trying to get them out of the drops... I lowered my protein concentration
