Hello all, I posted here a few months ago. My post dealt with a protein crystal that was very sensitive to oxidation (with the SeMet derivative even more so).
The protein grows in 24% PEG 1500, no more than 1 mM DTT, 0.05 M Hepes, pH 7.5, 1% glycerol. TCEP will not work, even at very low concentrations. Does anyone have any experience with soaking the crystals in high concentrations (10-50 mM?) of DTT or TCEP before freezing? Could this feasibly be used to "reverse" any protein oxidation right before freezing?
