[ccp4bb] cryo-EM

2018-08-31 Thread F.Xavier Gomis-Rüth
Dear all, is there some kind of general trend if not consensus as how to refine cryo-EM structures? What do people do for maps in the 3-4Å range, pure RSR against the untouched map and that's it? Reciprocal space refinement against the backtransformed structure factors from the map but with

Re: [ccp4bb] N-linked glycans

2018-04-20 Thread F.Xavier Gomis-Rüth
surprised to find such a thing in a crystal structure of a glycoprotein. What's the resolution of the data? On Fri, 20 Apr 2018 at 16:21 F.Xavier Gomis-Rüth <mailto:xgr...@ibmb.csic.es>> wrote: Dear all, has anybody ever seen a GlcNAc in boat conformation attached to an

[ccp4bb] N-linked glycans

2018-04-20 Thread F.Xavier Gomis-Rüth
Dear all, has anybody ever seen a GlcNAc in boat conformation attached to an asparagine and, if so, is there any reference for it? Thanks a lot in advance, Xavier --

Re: [ccp4bb] definition of "apo" and alternatives?

2018-01-31 Thread F.Xavier Gomis-Rüth
Hi Johannes, this is normal textbook knowledge, which got perverted over time and misusage. Just check with a serious dictionary like Merrian Webster or even the Wikipedia (see below). Best regards, Xavier https://www.merriam-webster.com/dictionary/apoenzyme https://en.wikibooks.org/wiki/S

[ccp4bb] Ikerbasque Research Fellows Call 2017

2017-02-20 Thread F.Xavier Gomis-Rüth
*Ikerbasque* would like to inform you that we have launched *a new international call* to reinforce research and scientific career in the Basque Country. We offer: * *15 positions for Promising ResearchersIkerbasque Research Fellows* o 5 year contracts o PhD degree between Jan200

[ccp4bb] Fwd: Re: [ccp4bb] comparison of different protein states

2017-02-01 Thread F.Xavier Gomis-Rüth
Dear all, just a side comment to Guillermo's and Debanu´s discussion, which deals with the term "apo". I got the text below from a reviewer, who—as an exception ;-)—was absolutely right: In enzymology, enzymes that require the aid of cofactors such as metal ions or prosthetic groups (e.g.

Re: [ccp4bb] Free Reflections as Percent and not a Number

2014-11-21 Thread F.Xavier Gomis-Rüth
As pointed out by a reviewer recently, this has been object of study in the past when Gerard K. and Axel B. found that actually 500-700 reflections should suffice to monitor Rfree with sufficient precision. I guess everybody will agree on that we want to have the best refined structure and this

[ccp4bb] Fwd: Re: [ccp4bb] molprobity clashscore, symmetry-related molecules?

2014-10-23 Thread F.Xavier Gomis-Rüth
This brings me a related issue with COOT: the spherical refinement does not consider symeqs either or does it? Xavier Original Message Subject:Re: [ccp4bb] molprobity clashscore, symmetry-related molecules? Date: Thu, 23 Oct 2014 14:53:53 +0200 From: Jose Manuel Du

[ccp4bb] Vacancy

2014-10-17 Thread F.Xavier Gomis-Rüth
*/Laboratory Technician in biochemistry/molecular biology/* A part-time position (50%) for a laboratory technician at FPII level or similar (Grupo Profesional 4 of CSIC) is available to work in the Structural MitoLab of the Molecular Biology Institute of Barcelona (IBMB) from the Spanish Resea

[ccp4bb] Postdoc in Barcelona

2014-09-15 Thread F.Xavier Gomis-Rüth
*/Postdoctoral position in protein biochemistry/molecular biology/* A senior postdoctoral position is available to work on peptidases, their inhibitors and their complexes. The hosting place is the Proteolysis Lab of the Molecular Biology Institute of Barcelona (IBMB) from the Spanish Research

[ccp4bb] Fwd: 20 Research positions - Ikerbasque

2012-09-06 Thread F.Xavier Gomis-Rüth
  Ikerbasque, the Basque Foundation for Science (Europe), would like to inform you that we have launched an international call to attract 20 senior researchers to the Basque Country (permanent p

[ccp4bb] Fwd: Re: [ccp4bb] Fwd: Postdoctoral vacancy in Barcelona

2011-10-17 Thread F.Xavier Gomis-Rüth
ing, or correct the link... Mark Mark J van Raaij Laboratorio M-4 Dpto de Estructura de Macromoleculas Centro Nacional de Biotecnologia - CSIC c/Darwin 3 E-28049 Madrid, Spain tel. (+34) 91 585 4616 http://www.cnb.csic.es/content/research/macromolecular/mvraaij On 17 Oct 2011, at 17:43,

[ccp4bb] Fwd: Could Biological Negative Results be published?

2011-07-11 Thread F.Xavier Gomis-Rüth
Dear CCP4ers, I think this is a very interesting initiative and it could potentially lead to a discussion within the board. Best, Xavier Mensaje original Asunto: Could Biological Negative Results be published? Fecha: Sun, 10 Jul 2011 21:39:52 -0500 De: David Alc

Re: [ccp4bb] Proper Reference for Phil Evans Program POINTLESS

2007-07-10 Thread F.Xavier Gomis-Rüth
Page, Rebecca escribió: CCP4ers: What is the correct reference for Phil Evans Program POINTLESS? Thanks for your help! Rebecca Evans, P. (2006) Scaling and assessment of data quality. /Acta Crystallogr. sect. D/, *62*, 72-82. --