[ccp4bb] refmac output query

, but I was wondering if there is a hidden problem somewhere, that might influence more crucial things. Many thanks, Emmanuel -- Dr. Emmanuel Saridakis Institute of Nanoscience and Nanotechnology National Centre for Scientific Research "DEMOKRITOS" 15310 Ath

Re: [ccp4bb] AlphaFold: more thinking and less pipetting (?)

binds where and in the way it is predicted to. So I daresay that it is an irreducibly experimental problem. Best, Emmanuel Dr Emmanuel Saridakis National Centre for Scientific Research DEMOKRITOS Athens, Greece - Original Message - From: John R Helliwell To: CCP4BB@JISCMAIL.AC.UK Sent: Tue

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

n. They are far superior and always have been." Emmanuel - Dr. Emmanuel Saridakis Principal Researcher Institute of Nanoscience and Nanotechnology National Centre for Scientific Research "DEMOKRITOS" 15310 Athens GREECE email: e.sarida...@inn.demokritos.gr From: "Bä

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

" I think women are foolish to pretend they are equal to men. They are far superior and always have been." Emmanuel - Dr. Emmanuel Saridakis Principal Researcher Institute of Nanoscience and Nanotechnology National Centre for Scientific Research "DEMOKRITOS" 15310 At

Re: [ccp4bb] Protein fold and the moonlighting function

I believe this is more than a fluke. It looks to me like a very powerful evolutionary tool which may have rendered the early stages in the evolution of life less improbable. Best wishes, Emmanuel Saridakis - Original Message - From: "Peter Stogios" To: "CCP4BB&qu

Re: [ccp4bb] crystallization optimization

Dear All, Fine-tuning protein and precipitant concentration is of course the first line of approach, followed by both rMMS and streak-seeding. I would like to remind you of a far less popular but often successful in my hands, optimisation technique: It consists in incubating the trial at the

Re: [ccp4bb] Alexander Rich

worse (occasionally). Emmanuel Dr. Emmanuel Saridakis PhD Biophysics, MSc History and Philsophy of Science Institute of Nanscience and Nanotechnology N.C.S.R. "Demokritos" Athens 15310 Greece - Original Message - From: "Edward A. Berry" To: CCP4BB@JISCMAIL.AC.UK Sent

[ccp4bb] 3D graphics linux compatibility question

tor such as the ASUS VG278H , which has an in-built nVidia 3D vision emitter which takes its stereo synch from a DVI cable is used, can we even use the simpler GeForce GTX670 or 680 ? As you might imagine, we are on a rather tight budget. Any info would be greatly appreciated! Emmanue

Re: [ccp4bb] Temperature and crystallization

lot more on the centeo.com site. Best, Emmanuel Saridakis - Original Message - From: Acoot Brett To: CCP4BB@JISCMAIL.AC.UK Sent: Monday, November 19, 2012 7:08 AM Subject: [ccp4bb] Temperature and crystallization Dear All, Will you please give a comment on

Re: [ccp4bb] do you think it is interesting?

Of course, "oligomer" (pure Greek) usually does that kind of job, but not in this specific case, since oligo means few and in this case we have "endless" chains. I can only think of the neologism "myriomer" for this particular case, if you want to stick to Greek. Myrioi can mean 1 or countle

[ccp4bb] Pointless problems

Dear All, I am having problems when trying to run Pointless with input from Denzo/Scalepack. (1) Pointless refuses to work from the .sca file. The error message is: CCP4 library signal mtz:File not identified as MTZ (Error) CCP4MTZfile: open_read - File missing or corrupted: rn7e222test.sca or

[ccp4bb] DTU vs DTT ?

Dear All, Possibly a trivial question but your experience would be much appreciated: I recently submitted a structure to PDB containing 3 DTT (dithiothreitol) molecules, or so I thought. The molecules had been imported and fitted with Coot using the Get Monomer... instruction with the code DTT.

Re: [ccp4bb] How to distinguish microcrystalline, quasicystalline and precipitation?

Hi Joy, Yes, I agree that it is very difficult to distinguish those and it is even more difficult to put it in words or in writing: experts have been talking about shininess, transparency (rather than brown colour) and birefringence as far as I know. All these methods have some grain of truth i

Re: [ccp4bb] Off-topic: hollow protein crystals

Dear Andre, Yes, hollow crystals are not very uncommon. We have recently also solved a structure at very high resolution using long hollow crystals. Initially the walls were very thin; we managed to make them thicker (and the hole correspondingly smaller) by standard fine-tuning of conditions.

Re: [ccp4bb] Setting Microbatch trays !!

a lot like a vapour diffusion experiment. Have a look at Douglas Instruments' web page: there is a lot of relevant info in there. Cheers, Emmanuel Saridakis - Original Message - From: rashmi panigrahi To: CCP4BB@JISCMAIL.AC.UK Sent: Friday, April 23, 2010 5:35 AM Subject: [c

[ccp4bb] PhD position in protein crystallography

PhD position in crystallization and structure solution of natural and appropriate variants of the sponge protein silicatein and nanobiotechnology applications TOPIC: Research in the field of biomineralization and applications in nanobiotechnology. Biomineralization is the formation of minera

[ccp4bb] Post-doctoral opening in macromolecular crystallography, Athens Greece

so eligible. HOW TO APPLY Applicants should forward electronically (a) their detailed CV (b) copies of published articles, (c) names, e-mails/phone numbers of two referees to: Dr. Emmanuel Saridakis or Dr Irene Mavridis es