Re: [ccp4bb] External: Re: [ccp4bb] AlphaFold: more thinking and less pipetting (?)

24-D2), as AlphaFold2 did not perform so well on the complete assembly. Deposited PDB is 6T1Z Cedric PS: I should also note that many other groups performed very well, much better than I would have dreamed, including on the full protein but just not as crazy-good. — Prof. Cedric Govaerts,

[ccp4bb] R/R free reported values

Hi Phenix and CCP4 community, (sorry for the cross posting). I was looking at a PDB file. The http://www.rcsb.org/ website page gives the following values: R-Value: 0.103 (work) R-Free: 0.134 The actual PDB file gives the following: R :0.110 FREE R VALUE : 0.170 I was wondering why the diff

[ccp4bb] cellulose and X-ray diffraction

. Thanks. -- .. Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherches Microbiologiques J.-M. Wiame -IRMW Campus CERIA - Av. E. Gryson, 1 B-1070 Bruxelles, BELGIUM e-mail: cedric.bauv...@ulb.ac.be tél: +32 (0)2 5273634

[ccp4bb] ADVX in remote acces is a nightmare

ys!), I can see the image once I've dragged another window onto the ADVX window, this seems to refresh it and then I can work with it fine. But as of now, that happens very only rarely... thus I cannot see my images.. Is there a trick/workaround ? Thanks... (beamtime is running by..) Cedric

[ccp4bb] Crystal Quick low profile 96 wells plate definition for Mosquito

Hi all, I'm looking for the plate definition for the Greiner CrystalQuick low profile 2 wells plates, flat bottom, for the Mosquito. I could not find the plate definition for the 2 wells... eagerly looking for it... Thanks ! Cedric Cedric Govaerts, Ph.D. Universite Libre de Brux

[ccp4bb] Measuring protein concentration with 2mM ATP around

(from one experiment to the next) rather than absolute value. I'm guessing as many of you have worked with ATPase etc, there must be a smart way to do this. Thanks for any input Cedric -- Cedric Govaerts, Ph.D. Universite Libre de Bruxelles Campus Plaine. Phone :+32 2 650 53 77 Building B

[ccp4bb] Postdoctoral position available : Crystallization of CFTR

Nanobody-aided crystallization and structure determination of CFTR Start date : march 1st 2013 The project: Cystic Fibrosis (CF or mucoviscidosis), is a fatal genetic disorder affecting one in 2500 newborn. It is caused by mutation in the CFTR, a chloride channel, leading to destabilization,

[ccp4bb] bioactive glass 45S5

. Cedric Bauvois Cristallographie des protéines Institut de Recherches Microbiologiques J.-M. Wiame -IRMW Campus CERIA - Av. E. Gryson, 1 B-1070 Bruxelles, BELGIUM e-mail: cedric.bauv...@ulb.ac.be tél: +32 (0)2 5273634 fax: +32 (0)2 5267273 ..

[ccp4bb] observations support

Dear All Does anyone know a software which can help to annotate observations in a crystallization experiment ? Thanks in advance, Regards Cédric -- .. Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherches

[ccp4bb] constrained monomer

Dear all, what are the best criteria to say that one monomer is more constrained by the packing than another? surfaces analyses by PISA? How to be sure that the observed differences are significant? Thanks in advance. Cedric Bauvois

Re: [ccp4bb] Angle between two different helices from two different subunits

helices of different subunits, after >> reading its logfile. I will appreciate suggesstions. >> >> >> Thanks in advance >> >> Peter >> > -- .. Dr. Cedric Bauvois Cristallographie des prot

[ccp4bb] off-topic: Compound search by chemical similarity

Dear All, Sorry for the off-topic question. Is there an easy way to search "chemically close" molecules and know if they are commercially available ? Many Thanks. -- .. Dr. Cedric Bauvois Cristallographie des protéine

Re: [ccp4bb] Summary: cryoloops for X-ray data collection from protein crystals at room temperature.

BM30/).*<http://en.wiktionary.org/wiki/commercialized> * contact: fer...@ibs.fr Best regards, Cédric -- Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherches Microbiologiques JM Wiame -IRMW Av E. Gryzon 1, 1070 Brussels (Belgium) tél: +32 (0)2 5273634 fax: +32 (0)2 5267273

[ccp4bb] cryoloops for X-ray data collection from protein crystals at room temperature

loop should be carefully chosen so that the crystal does not move in the cryoloop. This crystal mounting method can be time-saving compared to the traditional method to mount a crystal in a glass capillary tube. Many thanks -- Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherche

[ccp4bb] TLS parameter representation

Dear All, i would like to know wether it's possible to represent graphically TLS-tensors obtained via refmac5 and how to procede? Best Regards. Cédric -- Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherches Microbiologiques JM Wiame -IRMW Av E. Gryzon 1, 1070 Bru