hello all, I would like to thank everyone for all suggestions.
Summary: 1 - MiTeGen Tools - MicroRT™ Room Temperature Mounting System - crystals slipping ? (suggestion of juan: amongst other things, problem resolved by using micromesh loops instead of using standard loops) - uses polyester capillary which scatters xrays less than the glass - works quite well - everyting is reasonably airtight - much easier to do than the old way. links: http://www.jenabioscience.com/cms/en/1/catalog/733_microrttrade_room_temperature_mounting_system.html http://mitegen.com/products/micrort/micrort.shtml 2 - coat the crystal with paratone oil and mount the crystal in a standard cryoloop - no special tools required - you can use the same crystal to collect under cryo-conditions and directly compare the impact of cooling the crystal 3 - the free mounting system (FMS) from Proteros Biostructures: a humidity control device - it does not have highly volatile components in the conditions - room temperature not too difficult links: http://www.proteros.com/articles.php?sid=18&lang=de contact: kris.t...@rigaku.com 4 - the humidifier device HC1b - developed at the EMBL - available at ESRF - easy to use links: http://www.esrf.eu/UsersAndScience/Experiments/MX/special-setup technical information: weathe...@embl.fr 5 - test crystal directly on plate - japan http://journals.iucr.org/d/issues/2002/10/01/issconts.html http://www.labo.co.jp/contents/direct_ex.html contact: nobuh...@nagoya-u.jp - France, Grenoble explanations http://www.natx-ray.com/products/G-Rob_2D.html the movie http://www.natx-ray.com/products/G-Rob_2D_movie.html a prototype, commercialized by Natx-ray, is available on FIP beamline ( http://www.esrf.eu/exp_facilities/BM30/).*<http://en.wiktionary.org/wiki/commercialized> * contact: fer...@ibs.fr Best regards, Cédric -- Dr. Cedric Bauvois Cristallographie des protéines Institut de Recherches Microbiologiques JM Wiame -IRMW Av E. Gryzon 1, 1070 Brussels (Belgium) tél: +32 (0)2 5273634 fax: +32 (0)2 5267273
