Re: [ccp4bb] Trimming of carbohydrate chains

Dear Herman, Assuming that the protein is eukaryotic, and glycans N-linked, various ways of dealing with the issue are described in PMID: 17355862. Best wishes, radu -- A. Radu Aricescu, PhD MRC Senior Research Fellow Associate Professor University of

Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!

l if one could harness it! Best wishes, radu ------ A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-28

Re: [ccp4bb] Sighting of Protein Crystals in Vivo?!

ut of ~200 or more cells in the field? This does not look like transient expression using a normal plasmid... Very intriguing nevertheless! radu ------ A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Huma

[ccp4bb] Postdoctoral position, C2D2, University of York

ience/jobs/303788-Discipline-hopping-internships or http://www.york.ac.uk/c2d2/internships/ For informal enquiries contact: radu.caline...@york.ac.uk ------ A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Huma

Re: [ccp4bb] install crystallography software on mac

Best place to start: http://scottlab.ucsc.edu/~wgscott/xtal/wiki/index.php/Crystallography_on_OS_X -- A. Radu Aricescu, PhD University Research Lecturer University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology

Re: [ccp4bb] The effect of His-tag location on crystallization

Or indeed support surreal structures :-)) (PMID: 11853672 vs PMID: 14749821 and so on) -- A. Radu Aricescu, PhD University Research Lecturer MRC Career Development Award Fellow University of Oxford Wellcome Trust Centre for Human Genetics Division of

Re: [ccp4bb] mammalian expression vector

Hi Jerry, You may find that pcDNA3.1 won't give you the protein yields needed for crystallization. Have a look at PMID: 17001101 for an alternative. Your Kozak sequence looks good, radu ------ A. Radu Aricescu, PhD University Research Lecturer MRC C

Re: [ccp4bb] FW: [ccp4bb] highly glycosylated protein

, play with deglycosylation conditions etc) and produce a more homogeneous sample than CHOs (which are leaky, see Chang et al 2007, PMID: 17355862, for details). Best wishes, radu -- A. Radu Aricescu, PhD University Research Lecturer MRC Career

Re: [ccp4bb] Deglycosylation enzymes

bad deal in long term. Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom Phone: +44-1865-287564 Fax: +44-1865-287547

Re: [ccp4bb] update on SeMet production

2031) and many others followed. More recently, people have moved to transient expression and probably the easiest labelling method for HEK293 cells can be found in PMID: 17001101. Best wishes, radu ------ A. Radu Aricescu, PhD University of Oxford Wellcome T

[ccp4bb] OXION Postdoc fellowships and DPhil/PhD studentships

expression, purification and crystallization are warmly invited to apply :-) Best wishes, radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive, Oxford OX3 7BN United Kingdom

Re: [ccp4bb] Deglycosylation

truct design (eliminate Ser/Thr/Pro-rich domains), for other types have a look at http://www.prozyme.com/pdf/gk80110.pdf for example. Best wishes, radu ------ A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Divisi

[ccp4bb] Fwd: [ccp4bb] HEK293S

Dear Vaheh, I'm not aware of a commercial or cell bank source, but you can get these cells from HG Khorana's lab at MIT. Best wishes, radu ------ A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of

Re: [ccp4bb] insect expression system

Se-Met labelling and so on. Here are some references (PMID): 17355862, 17001101, 16823037, 11788735, 16082028. Radu -- A. Radu Aricescu, PhD University of Oxford Wellcome Trust Centre for Human Genetics Division of Structural Biology Roosevelt Drive