Re: [ccp4bb] CryoEM map refinements in coot

2024-12-18 Thread Krieger, James M
I guess you mean that you’ve done an initial global rigid body fit and you still want to do some refinement to get better local fits? Refmac servalcat is a good option and uses two half maps in MRC format. It comes as part of ccpem but does require ccp4 to be installed too Or alternatively, you

Re: [ccp4bb] CryoEM map refinements in coot

2024-12-18 Thread Paul Emsley
On 18/12/2024 18:17, tim smith wrote: Hi all, Can you please advise once we fit the map and open in coot. What is next step to do in coot. I am somewhat confused. Once you have done the fitting then you're done. Right? You can run your model through (another) refinement program before you a

[ccp4bb] CryoEM map refinements in coot

2024-12-18 Thread tim smith
Hi all, Can you please advise once we fit the map and open in coot. What is next step to do in coot. I tried refmac but that required ccp4 file which doesn't have. Please some one can help in elaborating and sharing any tutorial would be helpful. I'm new bie and first time I am trying model buildi

Re: [ccp4bb] Recommendations for crystallization screens

2024-12-18 Thread Ben Bax
Hi Saniya, Another place to start is molecular dimensions - they say for 'your initial crystallisation screen, please use our Screen Selection Guide and Screen Redundancy Guide to maximise the crystallisation chemical space sampled and increase your crystallisation success rate' - I have not tri

[ccp4bb] Two beamline scientist positions open for the Swiss Light Source 2.0

2024-12-18 Thread Leonarski Filip
Dear All, We have two openings for tenure-track beamline scientists in our group to develop software and methods for crystallography beamlines at the upgraded Swiss Light Source 2.0 synchrotron. The positions offer a unique opportunity to explore novel methods such as dynamic crystallography a

[ccp4bb] PhD positions at the International Max Planck School of Cellular Biophysics in Frankfurt, Germany

2024-12-18 Thread MPI-Biophys-PR
We are pleased to announce that the new application cycle of the International Max Planck Research School on Cellular Biophysics (IMPRS-CBP) is now open! We are looking for excellent students in natural sciences to apply for fully funded PhD positions in Frankfurt and Mainz, Germany. IMPRS-CBP