On 18/12/2024 18:17, tim smith wrote:
Hi all,

Can you please advise once we fit the map and open in coot. What is next step to do in coot.

I am somewhat confused. Once you have done the fitting then you're done. Right? You can run your model through (another) refinement program before you analyse and deposit - servalcat springs to mind.

I tried refmac but that required ccp4 file which doesn't have.

Refmac is part of the CCP4 Suite. You need CCP4 to get Refmac.

One can build Servalcat from source - https://github.com/keitaroyam/servalcat - and that doesn't need CCP4 - however I doubt that is easy for "newbies."

Please some one can help in elaborating and sharing any tutorial would be helpful. I'm new bie and first time I am trying model building.

There are some cryo-EM tutorial on my blog:

https://pemsley.github.io/coot/blog/

e..g this one:

https://pemsley.github.io/coot/blog/2022/08/27/Coot-Cryo-EM-Tutorial-Part-1.html

There are some tutorials on YouTube (e.g. sbgrid) I should make more.

Paul

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