Re: [ccp4bb] contour level for cryoEM deposition: coot vs chimera

2024-09-26 Thread Jon Cooper
Sorry, I think the answer is on Colin's reply. Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail Android Original Message On 26/09/2024 17:56, Jon Cooper wrote: > Another thing I am curious about is how a map can look good at 5.5 sigma > because an X-

[ccp4bb] Assistant/Associate Professor positions in structural biology at Univ. of Iowa

2024-09-26 Thread Schnicker, Nicholas J
The Department of Biochemistry and Molecular Biology at the University of Iowa College of Medicine is inviting applications for a tenure-track Assistant or Associate Professor position. Successful applicants will be able to establish an independent, extramurally funded research program probing b

Re: [ccp4bb] contour level for cryoEM deposition: coot vs chimera

2024-09-26 Thread Jon Cooper
Another thing I am curious about is how a map can look good at 5.5 sigma because an X-ray one probably wouldn't unless it was atomic resolution. Don't worry if there isn't a simple explanation. Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Sent from Proton Mail Android Original

Re: [ccp4bb] contour level for cryoEM deposition: coot vs chimera

2024-09-26 Thread Colin Palmer - STFC UKRI
Dear Anna, The absolute map value that Coot displays – i.e. the 0.115 in your “0.115 V = 5.5 rmsd” – is the raw value from the map file, and should be equivalent to the “Level” shown in the Volume Viewer in Chimera or ChimeraX (as long as the step value is set to 1). If you set the contour lev

Re: [ccp4bb] contour level for cryoEM deposition: coot vs chimera

2024-09-26 Thread Jon Cooper
Hello Anna I have no idea why the map format is unacceptable without more details of how you created the file. Presumably it is in ccp4 format, but there are others. Is it a binary file or humanly readable? It's EM so cell dimensions are artificial, etc, I think. Best wishes, Jon Cooper. jon.b

Re: [ccp4bb] Registration now open for CCP4 Study Weekend 2025 - Using software, AI and other methods to advance macromolecular models

2024-09-26 Thread Karen McIntyre - STFC UKRI
Some of you keen people may have noticed that the button to register is missing from the SW website!! Please use https://cvent.me/bxlPqr to register until it appears. Regards Karen McIntyre CCP4 Project Administrator CCP4 ED&I Champion UKRI-STFC Rutherford Appleton Laboratory Harwell Campus Di

[ccp4bb] Registration now open for CCP4 Study Weekend 2025 - Using software, AI and other methods to advance macromolecular models

2024-09-26 Thread Karen McIntyre - STFC UKRI
Dear all, Registration is now open for the 2025 CCP4 study weekend entitled " Using software, AI and other methods to advance macromolecular models". This year the CCP4 Study Weekend will be held as a hybrid event from the 7th to 9th January 2025, enabling people to choose whether to attend in-

[ccp4bb] contour level for cryoEM deposition: coot vs chimera

2024-09-26 Thread anna anna
Dear all, I am depositing my first cryoEM structure and I am facing the following problem concerning the contour level of the map. Briefly, where do I find the value for the contour level to associate to the uploaded map in Coot? Long story: I worked with coot and I am happy with my map when I se