Re: [ccp4bb] how to create a figure of a blob

2024-07-18 Thread Paul Emsley
On 7/18/24 15:33, Andrea Smith wrote: I have a “green blob” in my map and I want to create a picture of it. What is the best option to do this? [...] Is printscreen from Coot my only option? Density figures from Coot 1 can look, if I may be so bold, pretty good.  See, for example Catapano

Re: [ccp4bb] how to create a figure of a blob

2024-07-18 Thread David Briggs
Hi Andrea, Either CCP4mg or ChimeraX (with the Clipper plugin) can read .mtz files directly and create maps to output publication-quality blob pictures. Best, Dave Dr David C. Briggs CSci MRSB Principal Laboratory Research Scientist Signalling and Structural Biology Lab The Francis Crick

[ccp4bb] Principal Investigator CRYOEM@NOVA: STRUCTURAL BIOLOGY AND DRUG DISCOVERY LAB

2024-07-18 Thread Hartmut Luecke
We are pleased to announce an exciting opportunity for a Principal Investigator to join the NOVA University School of Science and Technology (Caparica, Lisbon Area, Portugal) as Leader of the Research Team, hired (on a permanent basis) under the ERA Chair project, CryoEM@NOVA, funded by the HORI

Re: [ccp4bb] how to create a figure of a blob

2024-07-18 Thread Roger Rowlett
I use ccp4 to create Fo-Fc or 2Fo-Fc omit maps, then read them into Pymol and display them with the isomesh command with an appropriate carve radius. That might pick up an unmodelled blob. __ Roger Rowlett On Thu, Jul 18, 2024, 10:33 AM Andrea Smith wrote: > Hi, > > > > I have a

[ccp4bb] how to create a figure of a blob

2024-07-18 Thread Andrea Smith
Hi,   I have a “green blob” in my map and I want to create a picture of it. What is the best option to do this?   Normally, to make figures of maps, I use pymol's "display ccp4 maps", but pymol shows maps around “site”. So if I don't fit anything into my blob, I can't create a “site” and displa

Re: [ccp4bb] Error in mosaicity estimation by iMOSFLM

2024-07-18 Thread Harry Powell
Hi The simple answer is to estimate your own value (as we always used to do before I coded this up about 20 years ago…) by overlaying a prediction over the images and determining what value of mosaicity gives the best coverage. It may also be worthwhile changing the “mosaic block size” in the “

Re: [ccp4bb] phenix.table_one issues

2024-07-18 Thread Andrea Smith
Hi,   I had recently the same issue. I restarted my system, repeated the task and then it worked. Best, Andrea On Thursday, July 18, 2024 03:42 CEST, Dale C <8378c932659c-dmarc-requ...@jiscmail.ac.uk> wrote:   Hi all, I am currently trying to generate a Table 1 in Phenix. I currently h