Dear All,
Multiple openings for postdoctoral fellows are available at the University
of Minnesota, USA, Department of Biochemistry, Molecular Biology, and
Biophysics, in the research group of MikaelElias
(http://www.eliaslab.org/ ) to work on NIH and NSF funded projects.
Postdoctoral Research Fellow Position Available: Biochemistry and Structural
Biology of Functional RNAs and RNA/protein Complexes A postdoctoral training
position is available in the laboratory of Yunsun Nam, in the Cecil H. and Ida
Green Center for Reproductive Biology Sciences at UT Southweste
Dear all,
We are proposing to significantly upgrade beamline I24 for Diamond-II to enable
improved dynamic and microfocus MX.
As part of this we will host a webinar on Tues 3rd November 16:00 – 17:00 GMT.
The webinar will comprise an introduction and overview from myself followed by
two short p
Dear Christian,
Kleywegt (1996; "Use of Non-crystallographic Symmetry in Protein
Structure Refinement." Acta Cryst D52, 842-857. DOI:
10.1107/S0907444995016477) reported that NCS-related molecules tend to
be similar but differences in ligand binding do sometimes occur (cited
Sevcik et al. 1996 in
Dear CCP4BB Members,
The new and fully-upgraded macromolecular crystallography beamline at CHESS,
FlexX, is now accepting proposals for the Spring 2021 run.
SPRING 2021 RUN: The regular proposal deadline for the Spring 2021 run is Oct.
28, 2020. However, we will accept late proposals due to Cov
Dear all,
the tenor seems to be, if you see the ligand in any of the
monomers, that's the mode the ligand binds. We have seen other cases,
though, too.
Usually the protein I am thinking of (TetR(D)) crystallizes with one
molecules /a.u. In solution it is an obligate dimer. The dimer is
forme
Hi Matthias,
Thank you for response. Could you provide one or more pdb codes as example?
Best,
Christian
*From:* Barone, Matthias
*Subject:* [ccp4bb] ligand bound to only one chain in the crystal
*Date:* Tuesday, October
Hi Christian
I wouldn't worry: if the density is clear that nails it. You didn't say
whether this is a soak or co-crystallization. I assume the former since
you mention solvent channels. In my experience this is much more likely in
the case of soaks, which can often (though not always) be ratio
Hi Christian
One of our proteins crystallizes always as non-crystallographic dimer. We
occasionally find inhibitors bound to a second non-canonical site. Usually, the
inhibitors bound to the second binding sites are sufficiently resolved only on
one of the protein dimer. In these cases I often f
Dear Christian,
We occasionally observe binding to only one monomer of a multimeric complex. I
don’t think this invalidates the biological significance of your finding. I
would superimpose the different monomers to see if they have (slightly)
different conformations that prevent ligand binding.
Dear Christian,
You obviously mean a _non-crystallographic_ trimer -- in a crystallographic
trimer the protomers (and their ligands) would be identical by definition.
On the other hand, chains related by non-crystallographic symmetry necessarily
differ in their environments. As a results of near
Hello,
In our structure only one chain in a crystallographic trimer
(non-biological) shows a ligand bound to it (with clear density). There
doesn't seem to be any channels (or lack of them) favoring that specific
site. Can the community give your opinion on whether this can make the
presence o
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