[ccp4bb] Two open positions in structural cell biology at UC-davis

Dear Colleagues, I am recruiting for two positions in my laboratory (http://microtubule.mcb.ucdavis.edu ) at the University of California, Davis. Our Laboratory is focused on structural mechanisms of molecular machines that regulate tubulin heterodimer bio

[ccp4bb] Prepare files with anisotropic B-values for deposition in i2.

In i2 if you try the 'Prepare files for deposition' to make the cif's, it seems to do a round of refinement, presumably to get the final stats, etc, but the anisotropic B-factors seem to be ignored and are lost from the output file, as far as I can tell. Hence, the R and R-free rise quite a bit.

Re: [ccp4bb] Figure of merit in refinement

Hi Fellows, I have tried to summarize these issues James raised, in consistent notation in Chapter 12 of BMC of which you can download an excerpt here: https://www.dropbox.com/s/crzwoa5lb8bk3x5/Pages_611-619_from%20BMC_rupp_ch12.pdf?dl=0 > if your R factor is 55% then your phases probably are

Re: [ccp4bb] Figure of merit in refinement

Hi James, I'm sure you realise this, but it's important for other readers to remember that the FOM is a statistical quantity: we have a probability distribution for the true phase, we pick one phase (the "centroid" phase that should minimise the RMS error in the density map), and then the FOM i

Re: [ccp4bb] Figure of merit in refinement

James - you do the most sensible informative tests! Thank you.. Eleanor On Fri, 4 Oct 2019 at 16:39, James Holton wrote: > I've done a few little experiments over the years using simulated data > where I know the "correct" phase, trying to see just how accurate FOMs > are. What I have found in

Re: [ccp4bb] Figure of merit in refinement

I've done a few little experiments over the years using simulated data where I know the "correct" phase, trying to see just how accurate FOMs are.  What I have found in general is that overall FOM values are fairly well correlated to overall phase error, but if you go reflection-by-reflection t

[ccp4bb] REMINDER closing date approaching: Protein Biochemist Position at Sosei Heptares, Cambridge UK

We are now seeking an experienced protein biochemist to join the Biomolecular Structure group at our research facility on Granta Park, Cambridge, UK, working on GPCR structure determination to enable drug discovery efforts. At Sosei Heptares we are equipped with state-of-the-art protein expression

Re: [ccp4bb] ITC question -dimer vs monomer

You may want to look at the following paper concerning Il8, dimerization, binding and ITC. http://m.jbc.org/content/279/35/36175.full Get Outlook for Android From: CCP4 bulletin board on behalf of Bernhard Rupp Sent: Friday, October 4,

Re: [ccp4bb] ITC question -dimer vs monomer

Thanks to All for the extended & informative responses. If true thermodynamic equilibria are realized, then I would agree that regardless of the pathway the endpoint (or integrated H) should be the same. The actual pathway and cooperativity probably will make this an interesting problem. I may