[ccp4bb] Faculty equivalent cryoEM position

** Faculty-Equivalent, Cryo-Electron Microscopy (Cryo-EM) Position Summary: As part of its program focused on advancing new measurement technologies and standards for biomol

Re: [ccp4bb] Re-using 96-well crystallization plates

Hello Nemanja, I used to wash and reuse glass plates for neutron crystallography. Of course glass is sturdier than polystyrene, but I can't think of any protein stain that would resist a treatment with detergent, then a strong base (say 0.1M NaOH) and finally a strong acid (say 0.1M HCl)... Regardi

Re: [ccp4bb] Re-using 96-well crystallization plates

Hi Nemanja, I have tried doing this, and it has never really worked for me, even with careful rinsing with MilliQ water after washing, I could never get well-shaped drops on a recycled plate. They are also a real pain to wash out, and it’s hard to get the last traces of protein out of the subwe

[ccp4bb] Call for Stanford-SLAC Cryo-EM Center (S2C2) Applications: Deadline June 1, 2019

Dear All, The second deadline for submitting proposals to the Stanford-SLAC Cryo-EM Center (S2C2) is coming up soon.Please apply at https://userportal.slac.stanford.edu/ by June 1. The missions of the Stanford-SLAC Cryo-EM Center (S2C2) are: 1. to provide access to state-of-the-art cr

Re: [ccp4bb] A Max Perutz question

Thank you to everyone who responded. I have a request in at both Nature (thanks Jonathan Davies and Daniel Bonsor for the article link) and also to the LMB (thanks to Harry Powell for helping me find the right person to talk to). A special thank you to my dear friend Savvas Savvides, who pointed

Re: [ccp4bb] Coot issue with add residue

Yes, Christian - that has happened to me. I found it by using the Validation Density fit., and noticing that a residue number which was not meant to exist had a Density fit bar. As you say there was a stray atom with that ID - totally incorrect - in the coordinate file.. Eleanor On Wed, 10 Apr

[ccp4bb] Re-using 96-well crystallization plates

Dear All, I'd like to ask if anyone has experience cleaning old 96 well crystallization plates? I have a large number of old plates (Swissci) with mostly INDEX and PEG Ion screens and I thought of re-using them instead of throwing them away, but I'm not sure if this would be viable. Best regar

Re: [ccp4bb] Coot issue with add residue

Well I think I recall one instance where it worked for me the way you describe. I think there was either a stray residue atom somewhere or a numbering not quite right. Could it be that this happens for you after autobuilding? Cheers On Wed, Apr 10, 2019 at 7:11 PM Hillen, Hauke wrote: > Dear al

Re: [ccp4bb] Coot issue with add residue

Dear all, Thank you for your many suggestions! Indeed, I was not able to fix the issue to my satisfaction. As Paul suspected, set_add_terminal_residue_do_post_refine was already set to 0. The triple-refine also did not improve anything. I also checked for the TER records, which I also suspected

[ccp4bb] ARP/wARP awk: Command not found.

Hi, auto_tracing.sh “ARP/wARP 8.0 patch 1”, after a few cycles, prints awk: Command not found., and exits a little later with: Data line -- RESOLUTION 20 2.2 Data line -- REMOVE ATOMS 8 CUTSIGMA 1.0 FORCE NEGATIVE MERGE 0.7 Data line -- FIND ATOMS CHAIN Z CUTSIGMA 3.2 RESN DUM T

Re: [ccp4bb] A Max Perutz question

You can also do a reverse image search with tineye https://www.tineye.com/search/153d902d2e4567ccb3299daefa3cef8d207d197a/ or google image search https://www.google.com/search?tbs=sbi:AMhZZiua3avdA9z9KnzBOe4pBpsmp-1LJjdEXd13gP1bdXWtunUl2CB6caY5wjn56t8GA8l9KNMqKZdLYHBYPpAuwanPvVp6nJImULD5vgQ9FtisQ

Re: [ccp4bb] A Max Perutz question

Hey Zac A good scan of page 228 in Georgina Ferry’s biography of Max Perutx (Max Perutz and the secret of life) will do the trick. It is a 18x13 cm photo. I have it in front of me! best Savvas > On 10 Apr 2019, at 16:29, Zachary A. Wood wrote: > > Hello Fellow Structural Enthusiasts, > > My

Re: [ccp4bb] A Max Perutz question

See below: https://www.nature.com/articles/449144a -- Jonathan Davies, PhD Postdoctoral Researcher, Stenmark Lab Department of Biochemistry and Biophysics Stockholm University Sweden On 10 Apr 2019, at 16:29, Zachary A. Wood mailto:z...@uga.edu>> wrote: Hello Fellow Structural Enthusiast

Re: [ccp4bb] A Max Perutz question

https://www.nature.com/articles/449144a Daniel A Bonsor PhD. Sundberg Lab Institute of Human Virology University of Maryland, Baltimore 725 W Lombard Street N370 Baltimore Maryland MD 21201 Tel: (410) 706-7457 From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Zachary A. Wood

[ccp4bb] A Max Perutz question

Hello Fellow Structural Enthusiasts, My apologies for the slightly off-topic question. I am trying to track down a higher resolution image of the jpg that I have attached. I use this photo of Max Perutz when I am teaching about protein folding, and have always wanted a better quality one. I bel

Re: [ccp4bb] [EXTERNAL] Re: [ccp4bb] High Rfree - ice ring

Slightly off topic, but a nice paper here. https://journals.iucr.org/m/issues/2019/03/00/tj5020/index.html The iceman cometh – but not to be confused with the play of the same name. Colin From: CCP4 bulletin board On Behalf Of Johan Turkenburg Sent: 09 April 2019 11:46 To: ccp4bb Subject: Re: