[ccp4bb] AW: [ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

Hi Roger, First, sigma is a relative measure. If sigma is very low, e.g. since your map contains 80% solvent, 3 sigma may correspond to the same absolute value e.g. in electrons/Å3 as 1 sigma in a standard map, so I would not be worried about that. However, an Rfree of 0.45 and a large off-orig

[ccp4bb] Industrial postdoc position at Biogen

Hi All, This is a job posting on behalf of a friend at Biogen, Cambridge, MA, US: We have a postdoctoral position open to study Wnt signaling and its role in neurodegeneration. A number of studies have shown the importance of the Wnt signaling pathway in the survival and maintenance of dopaminerg

[ccp4bb] Contouring 2Fo-Fc map, large blobs in Fo-Fc

Hi everyone, We have a structure that phaser found a solution for in P1, even though there is a large off-origin Patterson peak indicating pseudo NCS. However, the 2mFo-Fc map needs to be contoured up to >3 sigma to be reasonable, going lower makes it look like what I normally see at <1 sigma. If

[ccp4bb] Fwd: Structural Bioinformatics Training Workshop & Hackathon 2017 - Application of Big Data Technology and 3D Visualization

*Structural Bioinformatics Training Workshop & Hackathon 2017 - Application of Big Data Technology and 3D Visualization* San Diego Supercomputer Center/University of California, San Diego, June 26 - 28, 2017 *Application deadline: April 30, 2017.* https://www.etouches.com/mmtf2017 This 3-day ha

Re: [ccp4bb] Cryo-em model building with Balbes

Hi, MRC is a map format, and doesn't hold the reciprocal space structure factors that Balbes expects, and that are referred to in the error message. Yes, you will need to calculate structure factors, which are held in the MTZ format. Simplest way is to use Refmac, which deals with issues like

Re: [ccp4bb] Cryo-em model building with Balbes

phenix.map_to_structure_factors can do the conversion, and then all the crystallographic tools for model building should be ready to use. Tat Sent from my iPhone > On 25 Apr 2017, at 10:36 AM, KL Ho > <05c3a58110fa-dmarc-requ...@jiscmail.ac.uk> wrote: > > Dear All, > > We are interested

[ccp4bb] Cryo-em model building with Balbes

Dear All, We are interested to build an atomic model on a 3.7 A cryo-em map with BALBES-MOLREP pipeline. The map is currently in mrc format. It cannot be read by BALBES (the error message is structure factor file size exceed limit). I was wondering should I convert the mrc file to mtz format? Any

[ccp4bb] BioSAXS Essentials 7

Our annual BioSAXS Essentials training course is filling rapidly as usual. Only 8 seats left. Register now while there are still openings. http://meetings.chess.cornell.edu/BioSAXSEssentials/07/index.html ps: if all goes according to plan, it is looking like we will actually have *two full SEC-

[ccp4bb] Staff Scientist Position in the group of Kristina Djinovic-Carugo in Vienna

*Staff Scientist Position in the group of Kristina Djinovic-Carugo* * * *Staff scientist position*is available in the research group of Kristina Djinovic-Carugo in the Department for Structural and Computational Biology, Max. F. Perutz Laboratories at the University of Vienna, Austria. The

[ccp4bb] Institut Pasteur Course on Integrative Structural Biology 2017 : deadline extension

Institut Pasteur Course on Integrative Structural Biology Paris, July 17 – 22, 2017 ### Deadline extended to May 5 ### Dear all, We are pleased to announce the 2nd Pasteur Course on Integrative Structural Biology. Problems in structural biology today can very often not be solved by one t