Hi, MRC is a map format, and doesn't hold the reciprocal space structure factors that Balbes expects, and that are referred to in the error message. Yes, you will need to calculate structure factors, which are held in the MTZ format.
Simplest way is to use Refmac, which deals with issues like box size and map sharpening. See step 1) described at http://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/refmac/refmac_keywords.html#id.seab0879x62k More options for model fitting, building and refinement are on the CCP-EM page for the recent Icknield workshop: http://www.ccpem.ac.uk/training/icknield_2017/icknield_2017.php HTH m -----Original Message----- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of cheng tat cheung Sent: 25 April 2017 16:18 To: ccp4bb Subject: Re: [ccp4bb] Cryo-em model building with Balbes phenix.map_to_structure_factors can do the conversion, and then all the crystallographic tools for model building should be ready to use. Tat Sent from my iPhone > On 25 Apr 2017, at 10:36 AM, KL Ho > <000005c3a58110fa-dmarc-requ...@jiscmail.ac.uk> wrote: > > Dear All, > > We are interested to build an atomic model on a 3.7 A cryo-em map with > BALBES-MOLREP pipeline. The map is currently in mrc format. It cannot be read > by BALBES (the error message is structure factor file size exceed limit). I > was wondering should I convert the mrc file to mtz format? Any idea how to > convert the mrc to mtz? > > Many thanks in advance. > > Best wishes > Kok-Lian
