On behalf of Soichi Wakatsuki:
Dear all
We would like to invite you submit abstract to the structural biology session:
"Opportunities in structural biology with new light sources"
scheduled from 1:30PM to 5:00PM on May 28, 2014 as part of the American
Crystallography Association meeting in Alb
We invite you to submit an abstract to the
"Wavelengths and Particles as Tools in Structural Analyses"
session scheduled May 26, 2014, Monday morning during the ACA meeting in
Albuquerque. The focus will be on new applications and methods in X-ray and/or
neutron crystallography that explore t
Colleagues,
Many bacilli are known to have S-layer proteins outlining their surface and
such proteins are known to be glycosylated. I have a few questions below:
1) What's the signature motif of such glycosylation and what pathway is
involved?
2) Do you know any experts in this area, esp. in Pae
Dear colleagues,
We'd like to bring to your attention this year's 5th Murnau conference on
structural biology. This biennial structural biology meeting takes place in
Murnau, a picturesque village at one of the lakes south from Munich,
Germany. The meeting this year will be held from Sept 10
Dear CCP4bb
please forward to candidates that might be interested
http://uio.easycruit.com/vacancy/1125923/70931?iso=gb
best Preben
--
J. Preben Morth, Ph.D
Group Leader
Membrane Transport Group
Nordic EMBL Partnership
Centre for Molecular Medicine Norway (NCMM)
University of Oslo
P.O.Box 1137 Bl
UNL is for unknown ligand, while UNX is for unknown atom/ion.
And as Robbie said, UNK implies an amino acid of unknown identity.
http://www.wwpdb.org/documentation/format32/sect4.html
On 2/5/14, 1:38 PM, Robbie Joosten wrote:
Just to add to Matthew's good advice, UNK is the standard residue name
Dear all,
Our lab is predominantly interested in membrane proteins and currently
uses a Constant Systems device for E coli hooked up to a cooling unit
which pumps 4C water around the outside of the main chamber and keeps the
sample cool even when using large volumes. This works really well for E.
Just to add to Matthew's good advice, UNK is the standard residue name for
unidentified amino acids. Using it for your compound may cause conflicts in
other programs such as COOT.
Cheers,
Robbie
Sent from my Windows Phone
Van: Matthew Franklin
Verzonden: 5-2-201
Dear Tianyu,
I have found the recent review by:
Nagae and Yamaguchi in Int. J. Mol. Sci. 2012, 13, 8398-8429;
doi:10.3390/ijms13078398 quite a good starting point.
best
Savvas
On 05 Feb 2014, at 19:53, Gmail wrote:
> Dear colleagues, I am looking for structures of protein complexes in which
Starting with the density (or specific volume) of water, you can obtain
rho(water)=0.33 e-/A^3. It's a nifty little bit of arithmetic and dimensional
analysis.
-MW
Michael C. Wiener, Ph.D.
Professor
Department of Molecular Physiology
and Biological Physics
University of Virginia
PO Box 800886
Dear colleagues, I am looking for structures of protein complexes in which
N-linked glycans mediate inter-molecular protein-protein interactions. Can
anyone point me in the right direction? Thanks!
Tianyu
Gmail via foxmail
We always have used ice with the Microfluidics disruptor. We also, on occasion,
monitor the temperature of the process stream. I'm not convinced, at all, that
there is actually any problem with heating, but I thought it reasonable to put
the query out there. Thanks to all who emailed me.
-MW
O
Dear All,
Is there a way or program that is good at providing a solid estimate of F000
using the standard mean protein electron density of 0.433 e/A**3 and a mean
solvent electron density calculated from the crystallization conditions? I am
wondering how the latter might affect F000 if the con
Dear Colleagues,
I am pleased to announce the publication of the latest issue of the
Computational Crystallography Newsletter:
http://www.phenix-online.org/newsletter/
A listing of the articles and short communications is given below.
Please note that the newsletter accepts articles of a
If heat is a concern, you can place your Emulsiflex into a bin with lots of
ice. We have the version with cooling after lysis, but if you are paranoid you
can cool the whole thing.
It has the footprint of a 15” MacBook Pro but it’s way more expensive than that
:-)
Jürgen
..
Interesting 'off-topic' thread. I'm a rather long-time user of Microfluidics
cell disruptors (for E. coli or P. pastoris or S. ceresvisiae), and a
shared-use M110-P Plug and Play is used by most of the membrane-heads at my
place. I've generally been happy (or at least not unhappy) with it.
Howe
If there is a covalent link between CYS and UNK then it is better to create
that using jligand from ccp4.
There are nice tutorials designed by Andrey Lebedev. Tutorials are on this site:
http://www.ysbl.york.ac.uk/mxstat/JLigand/
Regards
Garib
On 5 Feb 2014, at 15:59, Ronnie wrote:
> I am as
Hi Ronnie -
Refmac has done the job for you (or for your friend). The Refmac log
file says that Refmac has created a file called .cif (the exact name
varies). You should open up that file and inspect it; it will be
Refmac's best guess about the geometry of that link. I usually specify
Dear Colleagues,
This is a reminder about the second CeBEM/CCP4 South American School in
Macromolecular Crystallography. The closing date for applications is
Monday the 10th of February.
This year's school will take place at Sao Carlos University in Brazil.
Full details including the program
I am asking this question on behalf of a friend: -Thanks!
Hi All - I am working at small biotech. We have
minimal budget for software, only CCP4 and Coot. We have a ligand that
binds covalently to cysteine. I define the parameter file using Prodrg in
CCP4 to get drug.cif file and then make a l
Dear Phoebe,
We have a constant systems homogenizer that we use routinely as a service
for researchers here at UGA. It is really easy to use and gets up to high
pressure (40k psi) so you can lyse plant cells or other difficult to lyse
cell types. You just pour/pump your resuspended cells, as low
Dear All,
I would be grateful if you could bring the enclosed/linked opportunity to the
attention of any suitable candidates in your institutions. Please also
encourage them to look at all the categories e.g. the computing category is a
bit of a misleading label (that might change soon).
The
Dear Shanti Pal Gangwar,
based on the information you give, this does not sound like "solved structure"
to me.
By the way, the ACA conference at the end of May will feature a session "data
processing with the pros", where difficult projects will be analyzed, and
hopefully structures like this
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