Dear Prem,
90-95% completeness is not great, but definitively acceptable und should not
stand in the way of solving your structure by MR.
I guess the XDS statistics you mention are from the highest resolution shell?
Otherwise they would be bad. To be sure you processed the data correctly, the
Dear Acoot,
if your metal is part of a protein crystal, your diffraction image still looks
like the diffraction of a protein crystal. If you see salt spots, your crystal
is salt, not protein.
However, what sometimes happens is that one has a badly diffracting protein
crystals with some tiny sa
Dear Prem,
If your protein has 2 domains, it is possible that they their relative
orientation is different in your target compared to the search model. Therefore
searching with the two domains separately can improve your Z-score in Phaser
and give you a better map.
However, you did not tell
Dear All,
The density obtained after molecular replacement using phaser at 2.5
Angstrom and then used buccneer for autobuilding of the model. I am not
getting reasonable R value (it is 38.5 %) but the figure of merit is 0.629.
As My protein has two domains. So is it possible to fragment the indiv
Dear All,
Suppose I have a crystal hit from the protein-metal complex, with the
possibility of that the hit is a salt crystal. When I diffract it by X-ray, I
got some metal (or salt) diffraction without the protein diffraction (maybe due
to too low protein resolution). Will you please tell me
Hi all,
Is there a special format (residue naming, atom naming, etc) for the heavy atom
file to assist buccaneer with sequence assignment? The one I have now seems to
be returning structures that do not have SeMet at these locations.
Thanks
F
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F
