Dear All, The density obtained after molecular replacement using phaser at 2.5 Angstrom and then used buccneer for autobuilding of the model. I am not getting reasonable R value (it is 38.5 %) but the figure of merit is 0.629.
As My protein has two domains. So is it possible to fragment the individual domain and then again perform the molecular replacement. How it will improve my phase more and how R-factor will be reduced ? Please help and direct me in proceeding in a right way, and if possible provide a protocol for doing that. Thank you With kind regards
