Dear Prem, 90-95% completeness is not great, but definitively acceptable und should not stand in the way of solving your structure by MR.
I guess the XDS statistics you mention are from the highest resolution shell? Otherwise they would be bad. To be sure you processed the data correctly, the low-resolution shells should have Rmeas around 5% or lower and I/sigma's of 20 or higher. As Klaus pointed out, 38% Rfactor after MR is not bad, but you probably first have to manually rebuild your structure before you can run Refmac. For a 2-domain protein, you may consider running Phaser with 2 separate domains. Best, Herman Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Prem Prakash Gesendet: Freitag, 29. November 2013 20:53 An: CCP4BB@JISCMAIL.AC.UK Betreff: [ccp4bb] Problem with R-factor, Completeness, and I/sigma I have processed my Data with XDS, here the completeness is 95.5% after merging two data sets of the same crystals (initially it was 90% when was not merged) at the scaling step and R-factor is 18.5 and I/sigma is 1.45. and I further went with this data for molecular replacement where the Z-value was found to be 13. and after running Refmac the Ractor was bad its about 38.4 % then I runned the bucneer for Autobuilding and the FOM gone higher from initial cycle 0.57 to 0.75 at resolution of 2.5 Angstrom. can anybody suggest me what should I do for bringing my R factor lower and at the better positon.
