Re: [ccp4bb] over-expression strategies

2013-09-13 Thread Phoebe A. Rice
Something I recently learned from a friend: try changing the 4th base in the coding sequence to G (e.g. ATGGxx...) and the ribosome will like it better. We tried it recently on a frustrating construct and it worked like a charm. From: CCP4 bulletin board [CCP4BB

Re: [ccp4bb] Adding ligand to crystallization drop

2013-09-13 Thread Roessler, Christian
Another thing to try if you want to "speed up" the soaking process is to optimize your crystal growth to give smaller crystals. 10-20 micron crystals can reach 80% occupancy in less than 30 seconds, at least in our very limited tests. But this quick soak method was important for our system as

Re: [ccp4bb] weekend puzzle

2013-09-13 Thread Paul Emsley
On 13/09/13 17:50, Andreas Förster wrote: Dear all, a structure I'm refining is enjoying a free R factor of 18 but still suffers from mystery density that nothing in the crystallization condition could explain. I can't explain it either. Maybe you like to venture some guesses? The missing

[ccp4bb] weekend puzzle

2013-09-13 Thread Andreas Förster
Dear all, a structure I'm refining is enjoying a free R factor of 18 but still suffers from mystery density that nothing in the crystallization condition could explain. I can't explain it either. Maybe you like to venture some guesses? The missing ligand is derivatized benzoic acid, nitrob

[ccp4bb] postdoctoral position in crystallography

2013-09-13 Thread Bing Chen
A postdoctoral position in x-ray crystallography is available immediately in Bing Chen’s laboratory at Boston Children’s Hospital/Harvard Medical School. A strong background in protein crystallography is required for working on challenging crystals of viral envelope glycoproteins. Previous expe

Re: [ccp4bb] video of crystal pick-up through microscope?

2013-09-13 Thread Swastik Phulera
Hi, May be this would help https://www.youtube.com/watch?v=QcsaWowulDM On Fri, Sep 13, 2013 at 2:51 PM, Frank von Delft wrote: > Hi all - does anybody know where I can find a video of a crystal being > harvested, but taken through a microscope? > > The Google has not been helpful, but I'm obvioul

Re: [ccp4bb] over-expression strategies

2013-09-13 Thread Fernandez, Elias J
Yes, the gene's been codon-optimized. The predicted mRNA structure appears stronger in the optimized version than the native, where it is high too. And, expression levels are unchanged. Elias From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Elias F

[ccp4bb] video of crystal pick-up through microscope?

2013-09-13 Thread Frank von Delft
Hi all - does anybody know where I can find a video of a crystal being harvested, but taken through a microscope? The Google has not been helpful, but I'm obviouly incompetent with these new-fangled things. Thanks! phx

[ccp4bb] Registration last day: "The Future of Microfocus Protein Crystallography"

2013-09-13 Thread Marjolein Thunnissen
Dear all, I would like to remind you on a workshop on "The Future of Microfocus Protein Crystallography" which will take place at the MAX IV User meeting on the 24th and the 25th of September, 2013, in Lund, Sweden. Recent developments within protein crystallography methods in combination with

[ccp4bb] Postdoc in Pearl Lab on Hsp90 Complexes - correction

2013-09-13 Thread Laurence Pearl
Postdoctoral Research Fellow in Structural Biology of Hsp90 Complexes (Fixed term) Ref 297 School of Life Sciences - Genome Damage and Stability Centre Full time, fixed term for 36 months Salary range: starting at £30,424 and rising to £36,298 per annum. Closing date for applications: 27 Septemb