Dear Tim,
I am sorry that you feel offended. It is rather unfortunate that you
got an impression from my secondary comment that I am asking for help
with pdbvconv when I wasn't. It is also rather unfortunate that I have
figured out what the problem was myself and therefore did not have an
opport
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Dear Ed,
I do feel offended because I follow the ccp4bb with the intention of
helping people with my answers, which does take time. If it turns out
the I wasted my time because of the lack of information I consider the
question asked not follow what I
Does anyone know of an expression vector (or really what I am interested in
is the existence of and/or sequence of a promoter/operator) for E. coli
protein expression that is NOT cAMP dependent and that is NOT a derivative
of the lac operon? Something like a pBAD or pPRO promoter mutant that did
N
On Wed, 2013-05-22 at 18:09 +0200, Tim Gruene wrote:
> the answers you received were correct with respect to the question you
> asked. If they are not satisfactory, you have not given sufficient
> information.
>
Tim,
Not sure when I expressed any dissatisfaction with replies I received.
I asked w
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Dear Ed,
the answers you received were correct with respect to the question you
asked. If they are not satisfactory, you have not given sufficient
information.
Regards,
Tim
On 05/22/2013 05:54 PM, Ed Pozharski wrote:
> Tim,
>
> naturally, the issue
Tim,
naturally, the issue is specific to a particular pdb file (it would be
indeed strange if the conversion tool from a major software package
would fail in general sense).
Ed.
On Wed, 2013-05-22 at 08:12 +0200, Tim Gruene wrote:
> Hi Ed,
>
>
> #> pdbvconv -p udo.pdb -o udov.pdb
> #> pdbvconv
*43**rd** Mid-Atlantic Macromolecular Crystallography Meeting*
*May 30 – June 1, 2013*
*Duke University*
*Durham, NC*
This is a reminder for the 43rd Mid-Atlantic Macromolecular Crystallography
Meeting, which will be held next week, May 30-June 01 at Duke University in
Durham, North Carolin
Theoretically? Millions to billions of years. Practically? They will
last until the first time you forget to fill the dewar. Or until the
Sun explodes and in the rush to leave Earth your descendants forget to
pack it. Whichever comes first.
Seriously, as long as the crystals have actual
in theory I would say decades/eons.
in practice it probably depends on how much water/ice your liquid nitrogen
contains, multiple refillings of the liquid nitrogen might slowly deposit tiny
ice crystals on the crystals and slowly make diffraction worse...
Mark J van Raaij
Lab 20B
Dpto de Estruc
careina
Cryocrystals will last several months and more. Make sure that your LN2
is dry.
On Wed, 22 May 2013 14:38:51 +0200, Careina Edgooms
wrote:
Hi
Does anybody know how long one could store a crystal in liquid nitrogen
for before it will no longer diffract well? I'm talking in the or
I dare say indefinitely. Your crystal life expectancy drops rapidly though with
somebody not remembering to fill up the dewar. Transferring crystals to pucks
etc. is another problem. I had some crystals which I could not remember what
they were (label broke off the cane) and we simply reshoot th
Hi
Does anybody know how long one could store a crystal in liquid nitrogen for
before it will no longer diffract well? I'm talking in the order of weeks to
months...
careina
Dear all,
I would like to announce a workshop on "The Future of Microfocus Protein
Crystallography" which will take place at the MAX IV User meeting on the 24th
and the 25th of September, 2013, in Lund, Sweden.
Recent developments within protein crystallography methods in combination with
the p
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