Re: [ccp4bb] What to put on Custom Declaration for shipped samples?

2012-11-06 Thread Frank von Delft
Can't say I've made Felix's experiences: I've never had problems and rarely funny looks -- a bit of patient explanation does the job. That's when flying with the dry shipper myself, so not quite what you asked; but I carry along a letter with nice departmental & university letter-head and im

[ccp4bb] Fwd: [ccp4bb] What to put on Custom Declaration for shipped samples?

2012-11-06 Thread Boris Brumshtein
May I add a little note: "Dewar" reads as a destructive device. "Thermos" reads as thermos, which the dewar is except for no relation to the Thermos trademark. Good luck, Boris Brumshtein -- Forwarded message -- From: Felix Frolow Date: Tue, Nov 6, 2012 at 1:32 PM Subject: Re:

Re: [ccp4bb] What to put on Custom Declaration for shipped samples?

2012-11-06 Thread Felix Frolow
Jim, dottore... Starting back traveling to synchrotrons in the beginning of 80 I say, do not volunteer information, more magic words you say, more papers you fetch, more faxes you send in advance more they will torture you. You do not need custom declaration anywhere (at least in Europe), in st

Re: [ccp4bb] What to put on Custom Declaration for shipped samples?

2012-11-06 Thread Sean Seaver
Hi Jim, If there are magic words that always get items through customs, please let me know! In our experience, it is very hard to predict what will or won't be held up. Lucky the vast majority of items go through without an issue. We've had better luck using FedEx instead of TNT shipping alth

[ccp4bb] What to put on Custom Declaration for shipped samples?

2012-11-06 Thread Jim Pflugrath
I was asked by our shipping folks what we should put on the Customs Declaration so that samples that we ship or that are shipped to us (in dewars, styrofoam boxes, and/or padded envelopes) would not be held up in Customs. I had them put: "Scientific samples of less than 1 mg of non-infectious

Re: [ccp4bb] how to find and add water molecules in electron density map in coot??

2012-11-06 Thread Nat Echols
On Tue, Nov 6, 2012 at 12:06 PM, saleem raza wrote: > I have to put water molecules in my model but It's difficult to judge that > electron density is for water of something else. How to differentiate > > How the electron density look like for metal ions like Ca and Na??? Sodium can't be dist

[ccp4bb] how to find and add water molecules in electron density map in coot??‏

2012-11-06 Thread saleem raza
I have to put water molecules in my model but It's difficult to judge that electron density is for water of something else. How to differentiate How the electron density look like for metal ions like Ca and Na??? regards saleem

Re: [ccp4bb] Coot 0.7 Saving Dialog opens behind main window

2012-11-06 Thread Sampson, Jared
I agree, this is a frustrating "feature." I tend to work around this by pressing Cmd-` (back-tick) to cycle through the X11 windows (this works for other applications as well), or by running Coot from within the directory containing the PDB file and using Ctrl-S to save with auto-incremented (e.

Re: [ccp4bb] PhD position Diabetes

2012-11-06 Thread Leonardo B. Federico
Hello, I would like if this job still is disponible and how do I apply. Thanls, Leonardo

Re: [ccp4bb] shelx c/d/e from ccp4i

2012-11-06 Thread Felix Frolow
In my hands shelxc/d/e pipeline as it is implemented in ccp4 is working perfectly. After slowing structure yesterday using hkl2map I resolved it using ccp4 to have faster access to buccaneer or ARP/wARP. Needless to say that I used data I have measured in the form of output.sca from SCALEPACK. S

[ccp4bb] shelx c/d/e from ccp4i

2012-11-06 Thread Tim Gruene
-BEGIN PGP SIGNED MESSAGE- Hash: SHA1 Dear all, since there were more than one remark about the shelx c/d/e interface in ccp4 6.3.0 crashing I just tried myself. I DO get output from shelxc, unlike e.g. Shanti Pal Gangwar who opened one corresponding thread on this bb, but shelxc crashes

Re: [ccp4bb] low resolution molecular replacement with partial model

2012-11-06 Thread Randy Read
Dear Daniel, There are two questions here. First, is there likely to be enough signal to get a clear solution for the molecular replacement; second, will getting a solution for the molecular replacement be of any use to you? On the first point, we have some new ways of estimating the signal of

Re: [ccp4bb] low resolution molecular replacement with partial model

2012-11-06 Thread Tomas Malinauskas
Dear Daniel, here is an example of MR at 7 A: http://www.pdb.org/pdb/explore/explore.do?structureId=4GZA http://www.ncbi.nlm.nih.gov/pubmed/23104057 Best wishes, Tomas On Tue, Nov 6, 2012 at 11:06 AM, Panne Daniel wrote: > Hi all, > > I was wondering if there are examples of successful MR with l

[ccp4bb] low resolution molecular replacement with partial model

2012-11-06 Thread Panne Daniel
Hi all, I was wondering if there are examples of successful MR with low resolution data (~7A). Our protein crystallized in the P21 (a= 111; b= 211, c=126; beta=99.7). We only have a partial search model (267 amino acids) that accounts for ~6% of the AU (however at 100% identity). Has anybody

Re: [ccp4bb] Copying R-free flags - possibly daft question.

2012-11-06 Thread Graeme Winter
Well there's a funny thing. It seems that this is already in xia2 (strange moment when I wade in there to make the change and found it already made, it seems from my logs in May) However, it seems that I have not actually released this version. My bad. An updated release will appear shortly. b

[ccp4bb] on NaCl and Tris for crystallization

2012-11-06 Thread Acoot Brett
Dear All, For the protein buffer for the crystallization purpose, if we buy from Sigma-Aldrich, which types of NaCl and Tris are much suitable? I am looking forward to getting your reply. Cheers, Acoot