[ccp4bb] Positions available - Photon Factory, Japan

Dear all The Structural Biology Research Center at the Photon Factory (Japan) is advertising for several PostDoc positions in structural biology. Notably, one position will link methodology in structural biology applied to XFEL by comparison with 'classical' x-ray sources at synchrotrons. Furt

[ccp4bb] microscope/imaging recommendations

I was wondering if anyone can recommend a good microscope/imaging system with a UV detection functionality to differentiate between protein and salt crystals. We are relatively new to crystal screening in our lab, so any additional hints and tips about the best all-round system (not high-throughpu

Re: [ccp4bb] Suggestion for storage buffer for protein

Hi Christine, I'm just now catching up on some of your older posts, so this may be too late too help. For Fab crystallization, my successful protein stabilization buffer contained 20-50 mM MES pH 6.0 or 6.5 with the minimum possible amount of salt to stabilize the protein - 50 mM NaCl, perhaps. Low

Re: [ccp4bb] Evaluating crystallogarphic experiment

Thanks for all the replies {off list}. Now it's wait and hope that this crystallization experiment is succesful.

Re: [ccp4bb] Changing HETATM to ATOM record

Unix command line: echo | pdbset XYZIN old.pdb XYZOUT new.pdb or pdbset XYZIN old.pdb XYZOUT new.pdb then Ctrl/D (or Ctrl/Z in Windows/DOS). otherwise default settings for pdbset in ccp4i. pdbset does all kinds of PDB file manipulations, including this one. Arguably changing HETATM -> ATOM sh

Re: [ccp4bb] Phaser and space groups

Hi, As of last week, this is a "known bug" (defined as one where I got around to adding it to the list on our Phaser Wiki). It has been fixed in the latest nightly builds of Phenix, and a new executable will be released through CCP4 in a few weeks, coordinated with the next stable release of P

Re: [ccp4bb] Changing HETATM to ATOM record

An alternative: vi my_pdb.pdb (carriage return) :%s/HETATM/ATOM /g (carriage return) :wq! (carriage return, or ZZ i.e. shift zz) This does the trick. Simple operations like this can be done easily with an editor. I don't know Windows editors (because I don't use Windows). F. > Dear all > > Is

Re: [ccp4bb] Changing HETATM to ATOM record

Hi, Something like this may do the trick: sed 's/HETATM/ATOM /' current.pdb > new.pdb Cheers Rob On 20 Aug 2012, at 16:14, Theresa Hsu wrote: > Dear all > > Is there any tool in CCP4 that can change all HETATM records in PDB file to > ATOM? I need the metals to be defined as ATOM for calcu

[ccp4bb] Changing HETATM to ATOM record

Dear all Is there any tool in CCP4 that can change all HETATM records in PDB file to ATOM? I need the metals to be defined as ATOM for calculation of its normal mode with elNémo. Thank you.

[ccp4bb] Phaser and space groups

Hi all, we have been running into a space group issue when we start phaser (2.5.1) through ccp4i since the installation of ccp4-6.3.0: We typically scale data in the point group and then let Phaser go through various space groups. Even though the corresponding line appears in the input file

[ccp4bb] Strange crystal morphology - needles with a periodic pattern

Hi ccp4bb community, apologies for the off-topic question but I hope someone in the community may have seen something similar before. I'm fighting to optimize some needles that don't currently diffract. After coaxing them into growth large enough to see their shape, I notice they have a funny morp

[ccp4bb] Senior Lecturing Position - University of Huddersfield

Dear all, The University of Huddersfield are advertising for a Senior Lecturing position in Biochemistry. Our X-ray facilities will consist of a new dual source (Mo and Cu), Bruker D8 Venture diffractometer with Photon-100 CMOS detector (to be installed end of 2012). We also have a Bruker Nanos

[ccp4bb] Postdoc positions in X-ray crystallography or electron microscopy at Imperial College London

Research Associates: London, United Kingdom Imperial College London Salary: £32,100 - £40,720 per annum Two Research Associate posts are available in the the research group of Professor Paul Freemont (www.msf.bio.ac.uk) in the in the Centre for Structural Biology, at the South Kensington Campus

[ccp4bb] two Postdoctoral positions at YSBL

Two Wellcome Trust funded postdoctoral positions are available at York Structural Biology Laboratory, to work with Dr Fred Antson. Both posts are available for 3 years, with potential to extend for a further 2-years. One project focuses on investigating protein-nucleic acid interactions within

[ccp4bb] PhD position available

A 4-year PhD position is offered at the Biomolecular Sciences and Biotechnology Institute, University of Groningen, the Netherlands. Starting date: position is open. Description The successful PhD candidate will determine X-ray structures of novel cytochrome P450 enzymes, to understand the str