Re: [ccp4bb] citric acid + 40%MPD (pHI -H1 condition)

2011-06-20 Thread Jacob Keller
I think you can get salt crystals from MgCitrate as well, so be forewarned! Jacob On Mon, Jun 20, 2011 at 6:55 PM, Nat Echols wrote: > On Mon, Jun 20, 2011 at 4:09 PM, Jayashankar > wrote: >> >> Should I be happy if i get my crystals with one of the condition with a >> commercial kit >> contain

Re: [ccp4bb] citric acid + 40%MPD (pHI -H1 condition)

2011-06-20 Thread Nat Echols
On Mon, Jun 20, 2011 at 4:09 PM, Jayashankar wrote: > Should I be happy if i get my crystals with one of the condition with a > commercial kit > containing citric acid and 40%MPD+ protein in(hepes+MgCl2)+ADP+VO3 > Citric acid will chelate magnesium - this may not disrupt the crystallization of th

Re: [ccp4bb] citric acid + 40%MPD (pHI -H1 condition)

2011-06-20 Thread Jürgen Bosch
It will freeze out of the drop. So that's good. Jürgen .. Jürgen Bosch Johns Hopkins Bloomberg School of Public Health Department of Biochemistry & Molecular Biology Johns Hopkins Malaria Research Institute 615 North Wolfe Street, W8708 Baltimore, MD 21205 Phone: +1-410-614-47

[ccp4bb] citric acid + 40%MPD (pHI -H1 condition)

2011-06-20 Thread Jayashankar
Dear friends, Should I be happy if i get my crystals with one of the condition with a commercial kit containing citric acid and 40%MPD+ protein in(hepes+MgCl2)+ADP+VO3 any insights ... S.Jayashankar Research Student Institute for Biophysical Chemistry Hannover Medical School Germany.

Re: [ccp4bb] Ghostview in CCP4 configure interface

2011-06-20 Thread Clemens Vonrhein
Hi Mar, are you using your own compiled version of CCP4? Or pre-compiled binaries? Maybe there is some 32/64-bit issue at play here ... I would expect your own compiled (on that machine) binaries to work best/correclty. But I'm just guessing ... Cheers Clemens On Mon, Jun 20, 2011 at 05:25:09

Re: [ccp4bb] Ghostview in CCP4 configure interface

2011-06-20 Thread Mark J van Raaij
Dear Clemens, All, when trying to open the .plt files I get "Bad plot84 file format" ... anyone has observed this and found a solution? xplot84driver is installed ("which xplot84driver" gives "/usr/local/ccp4-6.1.3/bin/xplot84driver") and the files appear ok, because I can convert them correctl

[ccp4bb] Summer X-rays at the NSLS

2011-06-20 Thread Robert Sweet
Summer X-rays at the NSLS The PXRR operates five beamlines at the NSLS: http://www.px.nsls.bnl.gov/. Two of these are the undulator beamlines X25 and X29. The dipole beamlines X12-B and X12-C are available, and are very effective for conventional crystallography. There are efficient ALS-Style

Re: [ccp4bb] Surface complementary output

2011-06-20 Thread Mike Lawrence
Hi Rojan The tables you refer to are histograms (see the documentation and the original paper), with the first two columns defining the left and right hand side bounds of each histogram bin. The first table is a histogram of the distances between the surfaces, with the area being that associated