On Mon, Jun 20, 2011 at 4:09 PM, Jayashankar <s.jayashan...@gmail.com>wrote:
> Should I be happy if i get my crystals with one of the condition with a > commercial kit > containing citric acid and 40%MPD+ protein in(hepes+MgCl2)+ADP+VO3 > Citric acid will chelate magnesium - this may not disrupt the crystallization of the protein, but it could result in a non-physiological active site. The coordination geometry of Mg2+ is hard to miss, so it's usually obvious whether it's there or not despite being the same number of electrons as water, but if you don't see a nice hexahedral arrangement, the citrate took it all. -Nat
