[ccp4bb]

Hi, well are you saying you are getting microcrystals with large (infite?) dilution of the precipitant or complex? at some point there must be change. try seeding? or then you are getting salt crystals for some reason? do you have a control drop? best of luck, tommi On Oct 11, 2010, at 8

[ccp4bb]

Thanks guys for your reply. This to clear the some doubt about my querry. 1.When I lowered the complex conc. then there are too many microcrystals.Same thing happen when I lower the precipitant conc. 2.My proteins pI is 6.45 and I am using a 50mM Tris pH 7.5 and 300mM NaCl 3.Before making compl

Re: [ccp4bb] Too many microcrystals.......

Hi Dilip- Try using your microcrystals as seeds and try the matrix microseeding method: Acta Crystallogr D Biol Crystallogr.2007 Apr;63(Pt 4):550-4. Epub 2007 Mar 16. An automated microseed matrix-screening method for protein crystallization. D'Arcy A

Re: [ccp4bb] Too many microcrystals.......

Hi, What is the pI of your protein? If it happens to be anywhere near your buffer pH, then you should move away from it. Have you measured the size of you micro-crystals? You may be surprised what micro-diffraction can do for you these days. Good luck! Nukri Ruslan Sanishvili (Nukri), Ph.D.

Re: [ccp4bb] Too many microcrystals.......

Hello Dilip Badgujar, What exactly do you mean by "not working" when you lowered to concentration? Did you lower the complex concentration and at some point you got - no precipitation at all - no crystals but aggregate precipitation - something else? You also do not mention that you tried loweri