That is a heavy PEG, and they arent such good cryo-agents. If you get
ice-rings when you test your buffer aone, try adding 15% of a much
lighter PEG (400 or 600).
Happy cryo-cooling
Elspeth
Roger Rowlett wrote:
With that much PEG in your mother liquor, I don't think you will need
any cryosolve
With that much PEG in your mother liquor, I don't
think you will need any cryosolvent. It will likely vitrify adequately
in LN2 directly from this solution. In solutions that do not contain
high concentrations of PEG or polyols, 25-30% glucose is nearly always
sufficient, and is compatible with
James Holton schrieb:
...
In any case, I think it is important to remember that there are good
reasons for leaving image file formats uncompressed. Probably the most
important is the activation barrier to new authors writing new programs
that read them. "fread()" is one thing, but finding the
There is another (at least partial) solution to this problem.
It is possible to write a routine to read a line in a free
format in which, in addition to items being separated by
'white space' and possibly commas etc., it is also assumed
that a minus sign cannot occur in the middle of a number s
