Re: [ccp4bb] cryosolvent

[Roger Rowlett]

With that much PEG in your mother liquor, I don't think you will need any cryosolvent. It will likely vitrify adequately in LN2 directly from this solution. In solutions that do not contain high concentrations of PEG or polyols, 25-30% glucose is nearly always sufficient, and is compatible with high salt concentrations. Cheers. rui wrote: Hi, All, What's the general rules for selecting cryosolvent?I got crystals in 30% PEG4000/PEG3350, 0.2M AS and 0.1M NaAC,how should I choose cryosolvent? Thanks. Rui -- Roger S. Rowlett Professor Department of Chemistry Colgate University 13 Oak Drive Hamilton, NY 13346 tel: (315)-228-7245 ofc: (315)-228-7395 fax: (315)-228-7935 email: rrowl...@colgate.edu