That is a heavy PEG, and they arent such good cryo-agents. If you get
ice-rings when you test your buffer aone, try adding 15% of a much
lighter PEG (400 or 600).
Happy cryo-cooling
Elspeth
Roger Rowlett wrote:
With that much PEG in your mother liquor, I don't think you will need
any cryosolvent. It will likely vitrify adequately in LN2 directly
from this solution. In solutions that do not contain high
concentrations of PEG or polyols, 25-30% glucose is nearly always
sufficient, and is compatible with high salt concentrations.
Cheers.
rui wrote:
Hi, All,
What's the general rules for selecting cryosolvent?I got crystals in 30%
PEG4000/PEG3350, 0.2M AS and 0.1M NaAC,how should I choose cryosolvent?
Thanks.
Rui
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