Re: [ccp4bb] selenomethionine labeled protein expression in insect cells

We have unsuccessfully used in the past (by that I mean less than stellar incorporation) the Gibco/Invitrogen Sf900-SFM w/o Met/Cys media. Then we saw Karin Reinisch's paper (Dong et al, Immunity, 2009) and they used Expression Systems' ESF921. This protocol works for us. Engin On 8/18/09 2:4

Re: [ccp4bb] selenomethionine labeled protein expression in insect cells

Hi Riya- Expression Systems has a protocol for SeMet incorporation (Click on the "Selenomethionine Incorporation" pdf file at http://www.expressionsystems.com/?mvcTask=documents) and they advertise that they do just about anything you would want to do with insect cells (see http://www.expressionsys

[ccp4bb] selenomethionine labeled protein expression in insect cells

Hi everyone, I am looking for a method for expressing selenomethionine labeled protein in insect cells. Can anyone point me to a suitable protocol ? Additionally, is anyone aware of companies that may provide this service for a fee ? Thanks

Re: [ccp4bb] Histogram matching in DM - question

Peter Grey wrote: Hi everyone, I am trying to use density modification at rather low resolution (4-5A ) for an RNA structure. My first time ever with RNA. I usually use Histogram matching as part of the density modification scheme in DM. But this method is based on density distribution of pro

Re: [ccp4bb] Histogram matching in DM - question

Hi Peter, Histogram matching works well for RNA structure, but be aware that density modification in general can be very tricky at low resolution. If by any means you can exploit averaging, e.g. multicrystal averaging of non-isomorphous crystals, it will probably be very helpful. It will

[ccp4bb] Histogram matching in DM - question

Hi everyone, I am trying to use density modification at rather low resolution (4-5A ) for an RNA structure. My first time ever with RNA. I usually use Histogram matching as part of the density modification scheme in DM. But this method is based on density distribution of protein maps I think. Is h

Re: [ccp4bb] imosflm with multiple data sets

Hi Hari, This is slightly different, and was indeed a bug in imosflm version 1.0.0. In the new version, released last week (1.0.3) this bug is fixed, so that if images from multiple sectors are used in indexing, the same matrix is defined for all those sectors (ie they will not be marked as U

[ccp4bb] SOMoRe

Dear CC4BB users, Does any one know how to get SOMoRe for molecular replacement? The old link and email to Diane Jamrog no longer functions. Also, does any one have a Mac Intel version? Best regards, Scott * Scott T. R. Walsh, Ph.D. C

Re: [ccp4bb] space groups not supported by Refmac5

Thank you all very much for your advice. I will reindex my data to P21212 in order to use Arp/Warp. Best regards, Arefeh Arefeh Seyedarabi, PhD Postdoctoral research assistant School of Biological and Chemical sciences Queen Mary, University of London Mile End road London E1 4NS Based at Joseph

[ccp4bb] 1-2 October: What is a Macromolecular Complex ? meeting at the NKI, Amsterdam

Dear all, This is a reminder and some news for the meeting: "What is a macromolecular Complex? Shades of Meaning Across Cellular, Systems, and Structural Biology" http://xtal.nki.nl/Oct2009 - Registration closes on Monday 7th September at 12:00 noon. No exceptions after the deadline. -

Re: [ccp4bb] Scale datasets from different crystals

I tried to scale the two datasets in scala after combining the two unmerged datasets with the sort MTZ program, but scala thinks the two datasets are from the same crystal. The problem is the unit cell constants are not identical. See scala output below. * Number of Datasets = 2 * Datase

Re: [ccp4bb] Protein Protein interactions

Hi, look in our servers list for interaction and contact analysis http://bip.weizmann.ac.il/toolbox/structure/tertiary.htm#contact pay attention to PISA which is very good for intermolecular and PIC for intra. Good luck Rotem On 17 Aug, 2009, at 20:13, protein.chemist protein.chemist w

Re: [ccp4bb] space groups not supported by Refmac5

Dear Arefeh, Arp/warp works with standard space groups, therefore you have to reindex P21221 to P21212. In this message from Anastassis Perrakis you can find some advice: https://www.jiscmail.ac.uk/cgi-bin/webadmin?A2=ind0709&L=CCP4BB&T=0&F=&S=&P=100954 Michele Arefeh Seyedarabi wrote: > Hi,

Re: [ccp4bb] Protein Protein interactions

Mariah, you may want to try PISA at http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html Eugene On Mon, 17 Aug 2009, protein.chemist protein.chemist wrote: Hello All, Can anyone tell me what are the programs used to find out the different interactions in a protein. I am talking about both int

[ccp4bb] Vacancy for crystallographer at Heptares

This is posted on behalf of Dr. Fiona Marshall at Heptares. The Heptares web site for applications is: http://home.btconnect.com/heptares/vacancies.html Please respond using the web site or the Email address below, not to me. Andrew Leslie Structural Biologist/Crystallographer Heptares is a

Re: [ccp4bb] imosflm with multiple data sets

Thanks Frank, Luke forgot to tell me that he had actually implemented this ! For those wishing to use this option, the "Matrix" line has to be dragged and "dropped" on the "Sector" line of the segment of data that you want to assign the matrix to, NOT to the Matrix line of that sector. I ag

Re: [ccp4bb] How to determine water number?

AntonioLeung wrote: I am a novice working on protein structure. When I pick water using COOT, too many waters picked, filling in the whole cell. "Too many" - hmm. Did you, by any chance, peak-search a difference map without first changing the default sigma cut-off to (say) 3.5? Paul.