Hi Peter,
Histogram matching works well for RNA structure, but be aware that
density modification in general can be very tricky at low resolution.
If by any means you can exploit averaging, e.g. multicrystal averaging
of non-isomorphous crystals, it will probably be very helpful. It will
also depend on the source of your phases
Poul
On 18/08/2009, at 21.43, Peter Grey wrote:
Hi everyone,
I am trying to use density modification at rather low resolution
(4-5A ) for an RNA structure. My first time ever with RNA.
I usually use Histogram matching as part of the density modification
scheme in DM. But this method is based on density distribution of
protein maps I think.
Is histogram matching still valid when it comes to RNA or protein/
RNA structures ?
And a general question regarding density modification and RNA
structure. Can statistical density modification programs (RESOLVE,
Pirate) take into consideration the chemical composition of the
structure ? Shouldn't this composition affect the expected density
distributions ?
My gratitude in advance for your comments and advice,
Peter.
