Peter Grey wrote:
Hi everyone,
I am trying to use density modification at rather low resolution (4-5A )
for an RNA structure. My first time ever with RNA.
I usually use Histogram matching as part of the density modification
scheme in DM. But this method is based on density distribution of
protein maps I think.
Is histogram matching still valid when it comes to RNA or protein/RNA
structures ?
I have the same question with respect to metalloproteins.
Presumably the heavier metal atoms make spikes that are completely off the
scale of a normal protein histogram. Is it then a bad idea to use
histogram matching? Do the metals get flattened down to the highest
density expected for protein on every cycle?
Ed
