Dear All,
assuming the chiral volume definition with r() a Cartesian position vector
Vc = [r(N)-r(Ca)]*([r(C)-r(Ca)]x[r(Cb)-r(Ca)])
I always get about -0.65 A**3
Is this correct? Are there any target values?
In a SHELX manual I read + 2.5 for the chiral volume - seems to
be different.
(not
what is wrong with NH4SO4?
It has a net negative charge.
The crystal always sticks with AmSO4.
Be happy it isn't decaying into plutonium.
lower the concentration of AS
Too much arsenic makes your coffee taste bitter.
Sorry to be the little-old-lady chemical nomenclature maven, but
How do you know your precipitate is AmSO4? At the low concentration youre
using, my guess is that the precipitate is protein.
It doesnt matter whether AmSo4 is a cryoprotectant or not (at 0.2 M, its not),
you have more than enough MPD and PEG400 in your solution to do the job.
Have you looked
I would think 30% MPD or more should normally be
enough to cryoprotect. If that is not the case, then you can always
supplement with additional MPD, glycerol, or glucose. If I understand
correctly, your crystals are forming in the presence of some additional
protein precipitate? If so, you migh
In the drop, the amSO4 becomes/casuses precipitate (I guess). The crystals were
grown from the precipitate within a few days. When I looped up the crystal the
precipitate was also looped up.
And I don't know whether AmSO4 is a cryoprotectant or not. When I freeze the
crystal in LN, the crys
Postdoctoral position at the MRC Laboratory of Molecular Biology,
Cambridge, UK (closing date April 17, 2009)
Dr Murray Stewart is seeking a postdoctoral researcher to work on the
structure and function of macromolecules involved in nuclear transport
and cell division. The Stewart group uses
On Apr 3, 2009, at 3:05 AM, Simon Kolstoe wrote:
2) To point imosflm to this version of Tcl/Tk the line "export
MOSFLM_WISH=/usr/local/X11/bin/wish8.4" needs to be in a file
called .bash_profile NOT .profile for some reason (but not if you
are using an X terminal in which case you need to
I also don't understand what you mean by "The crystal always sticks with AmSO4"
and "the crystal was still surrounded by AmSO4" Is there solid AmSO4 in the
drop? Judging from the concentration you state, this can't be the case.
Just loop up the crystal and freeze I would say, the MPD + PEG400
If you want to get rid of mother liquid around crystal, mount it in oil.
Howerver, what is wrong with NH4SO4? If your crystal can tolerate
near-saturated NH4SO4, it is a cryoprotectant all by itself.
-James Holton
MAD Scientist
HanJie_HCT Tai wrote:
Hi,
I have a protein-DNA complex grown
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium
sulfate/5% PEG 400/ 0.1M Tris (8.5).
...
I tried to grow crystal without AMSO4 or 0.1M AmSO4, but no crystals was
shown up.
So how would you test the following four hypotheses?
1. Sulfate is not required, ammonium is.
2. Ammoni
One way to do that is lower the concentration of AS form 0.2 to 0 or as
low as you can . This is depended on the xtal's stability . You can
transfer them directly or step by step.
Otherwise you can try to add water around the drop to increase the
humility or cover the drop by oil.
Good luck!
Hi,
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium sulfate/5% PEG
400/ 0.1M Tris (8.5).
I believe that 30%MPD above does not require any cryoprotectant but i hv a
problem to loop a crystal up. The crystal always sticks with AmSO4.
I tried to add 2ul up to 6ul reservo
Matt,
Your best bet would be to purchase the actual well condition from the
manufacturer. I know that both Hampton and Emerald can sell you a
larger quantity of what comes premixed in the screen. I am sure other
manufacturers can do the same. If you are lucky they might be able to
match the lot
CCP4 bulletin board wrote on 04/03/2009 03:58:35
AM:
>
> For example, the infamous 2HR0 entry,
(snip)
>
> Best - Tassos
>
I just wanted to point out that the structure in question is 2-H-R-zero
(which is what Tassos wrote), not the wholly innocent 2-H-R-O. Could we
perhaps encourage the PDB to
You are missing the line:
{xsize_pixel} {2048}
Am 03.04.2009 15:10, schrieb Scott Pegan:
I am attempting to process data taken at ESRF 14.1 using a Quantum 210
detector. I have tried all the def.site files I can find and tried
the binned switch, but the best I can get is the display seen in t
We often have good luck using protease cleavage sites as linkers. They're
evolved to have certain flexibility to them and they usually have a
healthy mixture of hydrophobic and hydrophilic amino acids. As a
delightful bonus they also offer the option to separate the two partners
at will.
Artem
>
Dear ccp4bb,
Thanks for the help. To summarise:
1) CCP4's tcl/tk installs into /usr/local/X11/bin
2) To point imosflm to this version of Tcl/Tk the line "export
MOSFLM_WISH=/usr/local/X11/bin/wish8.4" needs to be in a file
called .bash_profile NOT .profile for some reason (but not if you ar
On Thu, 2 Apr 2009, HanJie_Heng Chiat Tai wrote:
I have a crystal grown in 2.1M 1,6 hexanediol/0.1 M tro-sodium citrate (pH 6.5).
What's the cryoprotectant can be used to flash cool this crystal?
I recently grew crystals in 0.3M 1,6.hexanediol, cryoprotectant was PEG
400 (final conc. 32 %). P
I think Takanori is making here an extremely important point:
In each case, depositors can request to postpone the release against
the journal policy. It is not the wwPDB responsibility to force to
release data according to a journal policy. It is the depositor's
responsibility to follow the j
I recently had some success in this area. My approach was to get lucky
when I guessed the linker. In this case, my linker became ordered
which seemed to help my fusion construct settle down enough to exhibit
density. My linker sequence came was (approximately) alternating
hydrophilic and hy
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