If you want to get rid of mother liquid around crystal, mount it in oil.
Howerver, what is wrong with NH4SO4? If your crystal can tolerate
near-saturated NH4SO4, it is a cryoprotectant all by itself.
-James Holton
MAD Scientist
HanJie_HCT Tai wrote:
Hi,
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium
sulfate/5% PEG 400/ 0.1M Tris (8.5).
I believe that 30%MPD above does not require any cryoprotectant but i
hv a problem to loop a crystal up. The crystal always sticks with AmSO4.
I tried to add 2ul up to 6ul reservoir solution into the protein drop
(1+1 ul). Although AmSO4 was diluted, the crystal was still surrounded
by AmSO4.
The loop size is 0.1 -0.2mm, the crystal is about 0.1 mm.
I tried to grow crystal without AMSO4 or 0.1M AmSO4, but no crystals
was shown up.
Any suggestion to loop up the crystal without any AMSO4 attached?
Regards,
HCT (HanJie)
------------------------------------------------------------------------
Rediscover Hotmail®: Get quick friend updates right in your inbox.
Check it out.
<http://windowslive.com/RediscoverHotmail?ocid=TXT_TAGLM_WL_HM_Rediscover_Updates1_042009>
