One way to do that is lower the concentration of AS form 0.2 to 0 or as
low as you can . This is depended on the xtal's stability . You can
transfer them directly or step by step.
Otherwise you can try to add water around the drop to increase the
humility or cover the drop by oil.
Good luck!
leo
HanJie_HCT Tai wrote:
Hi,
I have a protein-DNA complex grown in 30 -40% MPD/0.2M Ammonium
sulfate/5% PEG 400/ 0.1M Tris (8.5).
I believe that 30%MPD above does not require any cryoprotectant but i
hv a problem to loop a crystal up. The crystal always sticks with AmSO4.
I tried to add 2ul up to 6ul reservoir solution into the protein drop
(1+1 ul). Although AmSO4 was diluted, the crystal was still surrounded
by AmSO4.
The loop size is 0.1 -0.2mm, the crystal is about 0.1 mm.
I tried to grow crystal without AMSO4 or 0.1M AmSO4, but no crystals
was shown up.
Any suggestion to loop up the crystal without any AMSO4 attached?
Regards,
HCT (HanJie)
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